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Questions related from Pranav Bhaskar
I am working on cloning and heterologous overexpression of terpene synthase enzyme. I have overexpressed and purified the enzyme. Now I want to characterize the enzyme by setting up the reaction...
01 June 2018 557 2 View
I did GC-MS for my samples. I got few new peaks which were absent in the substrate control. What could be the possible approach to analyze those peaks which may correspond to the novel compounds...
30 May 2018 3,101 4 View
I have purchased a highly volatile compound known as geosmin. It was contained in a glass ampule. Geosmin was dissolved in dichloromethane (DCM). I made several aliquots of 30Ul in a 1.5ml...
18 January 2017 5,915 3 View
I have purchased linear isoprene farnesyl pyrophosphate (as ammonium salt, dissolved in ammonia: methanol) and a bicyclic non-aromatic alcohol geosmin (dissolved in methanol). I am trying to find...
26 July 2016 3,727 3 View
I have transformed E. coli BL21(DE3) with the recombinant pET28a(+) containing the target gene. I had induced the culture in LB broth with 0.05 mM IPTG at 18oC for 18 hours and over-expressed the...
10 August 2015 6,431 4 View
Hello, I have got the purified recombinant enzyme and were eluted in 50mM sodium phosphate buffer and stored it in 15% glycerol at -20oC. The substrate for the enzyme is farnesyl pyrophosphate...
11 June 2015 2,588 4 View
I have cloned my gene in pET28(a) vector and transformed the recombinant plasmid into E. coli BL21 (DE3) and also in E. coli JM109 (DE3). I have checked the putativeness of the clones to be...
16 August 2014 8,500 7 View
