Pranav Bhaskar

4 Questions 9 Answers 0 Followers

Questions related from Pranav Bhaskar

How to analyze novel compounds by GC-MS?

I did GC-MS for my samples. I got few new peaks which were absent in the substrate control. What could be the possible approach to analyze those peaks which may correspond to the novel compounds...

30 May 2018 3,025 4 View

How to store geosmin, a highly volatile compound, after breaking the glass ampule?

I have purchased a highly volatile compound known as geosmin. It was contained in a glass ampule. Geosmin was dissolved in dichloromethane (DCM). I made several aliquots of 30Ul in a 1.5ml...

18 January 2017 5,782 3 View

How to determine volatile organic compounds (VOCs) dissolved in Methanol by Thin layer chromatography technique?

I have purchased linear isoprene farnesyl pyrophosphate (as ammonium salt, dissolved in ammonia: methanol) and a bicyclic non-aromatic alcohol geosmin (dissolved in methanol). I am trying to find...

26 July 2016 3,672 3 View

How to check whether the cloning is in-frame or not?

I have cloned my gene in pET28(a) vector and transformed the recombinant plasmid into E. coli BL21 (DE3) and also in E. coli JM109 (DE3). I have checked the putativeness of the clones to be...

16 August 2014 8,438 7 View