How can you attach polyurethane devices on glass slides?

I am making microfluidic devices made of polyurethane. The problem I am facing is that I am unable to attach these devices to glass slide even after plasma treatment (air plasma). Some of the...

28 January 2021 5,194 2 View

Is the assumption about Delaunay edge true?

01 July 2020 9,998 2 View

Lysis Buffer for RNA extraction?

Dear all, I am working on a magnetic beads based RNA extraction project. Presently, I managed to come up with a lysis to extract RNA from blood samples. However, my PCR results show inhibitory...

06 April 2020 9,953 3 View

Homing Receptors for tumor infiltrating T cells?

I am researching how different culture and expansion conditions contribute to a T cell's infiltration of a solid tumor. Was wondering what the best surface / intracellular receptors or ligands are...

26 June 2019 5,072 0 View

What is the source of basic 'hydroxyl' sites on gamma-alumina surface? Can hydroxyl ions form on its surface with only water present?

Consider an initially non-hydrated gamma-alumina surface. I pass water over the surface hoping it to dissociatively adsorb on the surface giving me hydroxyl radicals adsorbed on alumina surface....

31 March 2019 8,615 2 View

Does any one have a detailed protocol for isolation of Glucocerebrosidase from rat brain, please don't mind to share?

I am working on Gaucher"s disease, in this connection I would like to isolate GCase enzyme from rat brain. so please share the protocol if available. This isolation process should not include HPLC.

27 March 2019 2,591 1 View

Can anyone help me with my Horn Antenna project for 21 cm astronomy?

Me and a group of first year undergrads from my college are working on building a horn-antenna for radio astronomy similar to this...

16 April 2018 8,040 3 View

Can anyone suggest best possible resin for the Human Beta-2-Microglobulin purification from an albumin (60% - 80%) containing sample?

I have a feed containing B2M, albumin and antibody fragments from which i want to purify B2M as my sole target protein using Chromatography resin in one or two steps. If anyone has any good...

12 December 2017 9,312 3 View

Orthogonality of Asymptotic/WKB solution

It is known that the exact eigen-functions of sturm-liouville problem are orthogonal with respect to the weight function. But what happens when the solutions are obtained using approximate...

06 June 2017 486 3 View

How to store geosmin, a highly volatile compound, after breaking the glass ampule?

I have purchased a highly volatile compound known as geosmin. It was contained in a glass ampule. Geosmin was dissolved in dichloromethane (DCM). I made several aliquots of 30Ul in a 1.5ml...

17 January 2017 5,647 3 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

12% ledeburitic cold work tool steel, etched for revealing retained austenite, any help for clarifying?

Hello everyone. Given microstructure image belongs to a 12% ledeburitic cold work tool steel (annealed and tempered). The sample was etched with picral (100 mL ethanol and 4 g picric acid). I...

02 March 2021 4,119 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View