Say 1nm concentration?
Do we have to get short primary culture in a week,and do the all experiments ,how i sit psoobile,as more than a week ,tough to make cells survive from breast tumor.
Do we need to filter tumor after centrifuge to get cells adhered ,the cells are there,but with fat as well and after a week,media turns yellow?
So we have to pass cells through sieve ?
If you put IC50 of any cell line 15uM TAM in MCF-7 to create resistance, rather than going from the smallest concentration to the highest, is this a resistant cell line? In one month to three...
10 November 2013 5,058 1 View
The cells are flattened, adherent but debris is there so by the time they reach confluence, the debris is more. How to recover them, dmem phenol red free plus insulin am putting.
09 October 2013 7,109 9 View
What about p53 level in tam resistant cell lines with respect to parental mcf-7 cell line , is it the same or absent?
07 August 2013 4,444 0 View
Why is it difficult once the resistant cell line is made to revive the freezes? We can't just keep them running in culture, and if we change medium like from phenol red to phenol red free then to...
06 July 2013 474 11 View
How to reduce background in Western blot, per antibody for overnight at four degree or 1 hr at RT,then ,blocking 1 hr . Can I prolong blocking for 2 hrs,then wash with 1x pbst 5 mts each an dlast...
04 May 2013 2,003 9 View
I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...
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I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...
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Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...
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Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?
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We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...
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I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....
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Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...
01 March 2021 8,169 2 View
I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...
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Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.
28 February 2021 7,883 3 View
