Shazia al

6 Questions 28 Answers 0 Followers

Questions related from Shazia al

If you put IC50 of any cell line 15uM TAM in MCF-7 to create resistance, is this a resistant cell line?

If you put IC50 of any cell line 15uM TAM in MCF-7 to create resistance, rather than going from the smallest concentration to the highest, is this a resistant cell line? In one month to three...

11 November 2013 5,085 1 View

For a primary culture of a cell line from a breast tumor, do we require estradiol in the culture as well?

Say 1nm concentration?

11 November 2013 3,849 3 View

Why is there debris in tam resistant mcf-7 cell lines after a year?

The cells are flattened, adherent but debris is there so by the time they reach confluence, the debris is more. How to recover them, dmem phenol red free plus insulin am putting.

10 October 2013 7,137 9 View

Is P53 in tam resist cell line with respect to parental mcf-7 cell line the same or absent?

What about p53 level in tam resistant cell lines with respect to parental mcf-7 cell line , is it the same or absent?

08 August 2013 4,481 0 View

Need help with tamoxifen resistant cell line, mcf-7 at ic 50 15uM.

Why is it difficult once the resistant cell line is made to revive the freezes? We can't just keep them running in culture, and if we change medium like from phenol red to phenol red free then to...

07 July 2013 505 11 View

How to reduce background in Western blot?

How to reduce background in Western blot, per antibody for overnight at four degree or 1 hr at RT,then ,blocking 1 hr . Can I prolong blocking for 2 hrs,then wash with 1x pbst 5 mts each an dlast...

05 May 2013 2,031 9 View