Mycolplasma? But i need these cells badly and hw to check for mycoplasma

Hw will i recover the cells

Moreover they dont resemble in shape as mycoplasma,debris smallparticles not moving all over cells.

you can remove debris by different centrifugations. In my study, centrigation is done at 500 rpm 10' and there was no debris.

Ya i am doing same centrifuge same rpm and time it works but again the debris comes bk once theyreach confulency

Thanks shiv i will try to do that

In fact what if i can treat with antibiotic anti-mycotic fungizome in my m eida as recommended .5 % for 20x

Ok thankyou so much

I need these cells badly,i cant throw them,i used anti-mycotic yes,cell number was less but it cleared rest bit od debris,hopefully by pbs wash it will be clean.

I will have to work with mycoplasma protocol.let me observe cells.thanks