Is P53 in tam resist cell line with respect to parental mcf-7 cell line the same or absent?

Shazia al @Shazia_Al

08 August 2013 0 5K Report

What about p53 level in tam resistant cell lines with respect to parental mcf-7 cell line , is it the same or absent?

Badges
Science topic

More Shazia al's questions See All

For a primary culture of a cell line from a breast tumor, do we require estradiol in the culture as well?

Say 1nm concentration?

10 November 2013 3,866 3 View

If you put IC50 of any cell line 15uM TAM in MCF-7 to create resistance, is this a resistant cell line?

If you put IC50 of any cell line 15uM TAM in MCF-7 to create resistance, rather than going from the smallest concentration to the highest, is this a resistant cell line? In one month to three...

10 November 2013 5,104 1 View

Why is there debris in tam resistant mcf-7 cell lines after a year?

The cells are flattened, adherent but debris is there so by the time they reach confluence, the debris is more. How to recover them, dmem phenol red free plus insulin am putting.

09 October 2013 7,159 9 View

Need help with tamoxifen resistant cell line, mcf-7 at ic 50 15uM.

Why is it difficult once the resistant cell line is made to revive the freezes? We can't just keep them running in culture, and if we change medium like from phenol red to phenol red free then to...

06 July 2013 528 11 View

How to reduce background in Western blot?

How to reduce background in Western blot, per antibody for overnight at four degree or 1 hr at RT,then ,blocking 1 hr . Can I prolong blocking for 2 hrs,then wash with 1x pbst 5 mts each an dlast...

04 May 2013 2,056 9 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View