For a primary culture of a cell line from a breast tumor, do we require estradiol in the culture as well?

Say 1nm concentration?

10 November 2013 3,818 3 View

If you put IC50 of any cell line 15uM TAM in MCF-7 to create resistance, is this a resistant cell line?

If you put IC50 of any cell line 15uM TAM in MCF-7 to create resistance, rather than going from the smallest concentration to the highest, is this a resistant cell line? In one month to three...

10 November 2013 5,058 1 View

Why is there debris in tam resistant mcf-7 cell lines after a year?

The cells are flattened, adherent but debris is there so by the time they reach confluence, the debris is more. How to recover them, dmem phenol red free plus insulin am putting.

09 October 2013 7,109 9 View

Need help with tamoxifen resistant cell line, mcf-7 at ic 50 15uM.

Why is it difficult once the resistant cell line is made to revive the freezes? We can't just keep them running in culture, and if we change medium like from phenol red to phenol red free then to...

06 July 2013 474 11 View

How to reduce background in Western blot?

How to reduce background in Western blot, per antibody for overnight at four degree or 1 hr at RT,then ,blocking 1 hr . Can I prolong blocking for 2 hrs,then wash with 1x pbst 5 mts each an dlast...

04 May 2013 2,003 9 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View