I am trying to use DynaMut2 to predict a change in protein TBX4 after a mutation. please can someone help me that from where I can take information about the chain of my protein? i need to enter this information in DynaMut2?
I got comment on my FTIR data figure from a reviewer. The reviewer said "FTIR data in Figure should be repeated. there is no bassline." I made Y off set comparison graph of FTIR on OriginLab. Can...
03 August 2024 6,070 3 View
I am looking for journals that admit the publication of results from applying the IUCN Standard for designing and monitoring Nature-based solutions. Many thanks for considering my request.
23 July 2024 6,444 2 View
Hello, I want to load Remedesivir (RNA polymerase inhibitor) into mesoporous nanoparticles (MSNs). The issue is drug is temperature and pH sensitive and it degrades quickly. I need advice on its...
01 July 2024 889 3 View
Hello I have prepared metal nanoparticles and did zeta potential. Normally there is a single peak in sample but now there are 3 peaks. I prepared sample for analysis first I prepared 10 mg/mL...
30 June 2024 1,063 4 View
When I examined the GIXRD data obtained recently, I observed that the sample treated with O2 plasma on the Cu surface deposited on Si chips showed increased intensity compared to the sample...
19 June 2024 6,900 5 View
I am working on an acetyltransferase that is highly unstable. Its pI is 6.65, and its molecular weight is around 18 kDa. The protein elutes at 1M imidazole and begins to precipitate immediately...
19 June 2024 2,590 0 View
I conducted GIXRD measurements after performing O2 plasma treatment on a Cu surface. It was observed that the baseline is significantly high at low angles (10-30 degrees). What could this...
18 June 2024 2,996 3 View
I attempted to model a temporary 'lane closure' due to an accident or work zone. Initially, I successfully closed Lane 1 on Link 1, and it worked as expected. However, when I tried to close Lane 1...
17 June 2024 2,070 0 View
Hello guys. I have faced the problem of being in need of carboxyl-methylated amino acids. Amino acids I need are not available in the market so I need to synthesize them by myself. So thus I'm...
16 June 2024 9,920 3 View
I found a variant segregating with a genetic condition, this variant is listed in ClinVar (with no clinical significance), gnomAD, etc. However, I could not find any publication reporting the...
10 June 2024 7,606 3 View
Hey! I aim to generate a transgenic knockin zebrafish line that mimetizes a genetic condtition that leads to a certain disease on human. To do so, I need to insert a codon for mutagenic aminoacid...
14 July 2024 6,240 0 View
Studying a specific monogenetic disease based on mutations in different individual genes. I try to recreate the disease phenotypes of donors in vitro. I knocked out a gene x with above 90%...
12 July 2024 6,313 0 View
I have done site-directed mutagenesis (Substitution) to obtain a Cysteine mutation in my gene of interest. Now, I have two domains in my protein, And I want to delete one of the domains to obtain...
05 June 2024 9,430 1 View
Now, I am using CRISPR/Cas 9 system to make a point mutation at THBD gene. I am wondering how can I isolate the desired cell population after making a point mutation by using CRISPR/Cas 9? Are...
30 May 2024 9,993 0 View
We want to suppress self proteolysis of a protein. What is the strategy to modify the sequence in order to make it stable? The site is SLG - NX and SLG - AX.
25 May 2024 7,649 0 View
I am using GraphPad prism for data visualisation and analysis. I am trying to assess if there is a statistically significant difference between the percentage of 2 cohorts (patients and cell...
17 May 2024 4,424 1 View
I am working on a project to detect genetic mutations in lung cancer using machine learning algorithms and I am new to genetics and medical sciences. Can I use RNA as input to the algorithm for...
13 May 2024 5,726 6 View
This is from an endomyocardial biopsy from a 24 year old male with clinical hypertrophic cardiomyopathy. However, no mutation is found in the related genes. What are the irregular nuclear-like...
12 May 2024 8,148 1 View
presently I'm doing one study on ASSOCIATION OF COMT GENE VARIANT WITH ALCOHOL USE DISORDER. the methodology consists of conventional pcr with comt primers and rflp with NIA III rest enzyme. after...
22 April 2024 4,927 3 View
Hi lentivirus experts, I have lentivirus plasmids that carry mutations in the 3' and 5' LTR region. These regions were not exactly designed for easy cloning. Therefore, I wonder if it is possible...
14 April 2024 3,054 2 View