24 July 2024 2 6K Report

I am looking for journals that admit the publication of results from applying the IUCN Standard for designing and monitoring Nature-based solutions. Many thanks for considering my request.

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Pink bacterial colonies?

I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...

22 July 2024 5,953 6 View

Cell proliferation assays suggestions?

What cell proliferation assay do you suggest? cyQUANT from thermo Fischer has good reviews, a lot of good articles for the kit from Abcam. and Promega kits look easy and straight forward.

21 July 2024 1,554 2 View

How do you assign allele numbers and sequence type after amplifying housekeeping genes by PCR for MLST?

How to assign allele numbers and sequence type after amplifying housekeeping genes by PCR for genotyping of S. pneumoniae strains by MLST using bioedit ?

19 July 2024 5,239 2 View

How to make chocolate milk ?

we want to produce chocolate milk industrally .Dose anyone have a specific stabilizer formula for this pourpose?

10 July 2024 3,791 1 View

What does an increase in intensity mean in GIXRD?

When I examined the GIXRD data obtained recently, I observed that the sample treated with O2 plasma on the Cu surface deposited on Si chips showed increased intensity compared to the sample...

19 June 2024 6,900 5 View

Challenges in Stabilizing and Concentrating a Highly Unstable Acetyltransferase for Crystallization?

I am working on an acetyltransferase that is highly unstable. Its pI is 6.65, and its molecular weight is around 18 kDa. The protein elutes at 1M imidazole and begins to precipitate immediately...

19 June 2024 2,590 0 View

What could be the reason for the increase of baseline at low angles during GIXRD measurement?

I conducted GIXRD measurements after performing O2 plasma treatment on a Cu surface. It was observed that the baseline is significantly high at low angles (10-30 degrees). What could this...

18 June 2024 2,996 3 View

Vissim "lane closure" works well on "first" link, but not working on "second" link?

I attempted to model a temporary 'lane closure' due to an accident or work zone. Initially, I successfully closed Lane 1 on Link 1, and it worked as expected. However, when I tried to close Lane 1...

17 June 2024 2,070 0 View

Methods of O-methylation of amino acids without interfering the amine groups?

Hello guys. I have faced the problem of being in need of carboxyl-methylated amino acids. Amino acids I need are not available in the market so I need to synthesize them by myself. So thus I'm...

16 June 2024 9,920 3 View

About mineral exploration?

Are there any techniques at ore exploration that could replace the compass-tape measure of mineralized structures?

14 June 2024 3,254 2 View

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