We want to suppress self proteolysis of a protein. What is the strategy to modify the sequence in order to make it stable? The site is SLG - NX and SLG - AX.
Dear all - I'm trying to analyze hydrophobic patches on protein structures towards their size area. I have read previous postings and found some servers that would do such calculations. However,...
12 December 2023 7,381 0 View
Dear all - we would like to produce and study biofilms of Pseudomonas aeruginosa. We are not well equipped, only a simple microscope. I wonder if it could be possible to make estimations on the...
03 October 2023 2,578 2 View
Dear all, I want to use Alphafold to run this program on my labtop or a server which we will install rather soon. At the moment I run AF using Colab and this work well. But in the future I will...
16 November 2022 4,591 1 View
Dear all - I'm trying to understand if it might be an idea to treat Asthma by using small molecules produced by bacterial microbiomes. I read that several small molecules such as Ac, Propionate...
18 November 2020 7,544 2 View
Dear all - I would like to see the atomic displacement of two protein structures e.g. one with and the other without a ligand or domain and plot this on the sequence. Could you let me know how you...
12 April 2020 6,703 0 View
Are there any data/program/server available to predict/approximate the running behaviour (peak retention) of a needle like protein (7 x 2 x 2 nm) on a size exclusion column. Thanks and best...
29 January 2017 1,946 6 View
If there was a peptide that binds specifically to a folded protein, can it also bind to the same unfolded protein? Would it be logical to assume that a peptide can generally bind more strongly...
01 January 1970 989 6 View
Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.
07 August 2024 8,782 3 View
Dear readers, Thanks for your attention. I am wondering about the health economic problem of quantifying the value of interventions which a) prevent, b) improve symptom profile and c) ultimately...
05 August 2024 3,246 1 View
I am attempting to conjugate PEG to an amino acid at the C-terminus, for the purposes of producing nanoparticles. I have been told that PEG modified with amine groups can be used for this purpose,...
31 July 2024 2,033 1 View
Hi, researchers, Is it possible to use a safer acid instead of monochloroacetic acid to convert bacterial cellulose to carboxymethyl cellulose?
29 July 2024 4,570 0 View
I am currently performing cell-free DNA (cfDNA) extraction from plasma using an in-house developed kit that employs silica-coated magnetic beads. My protocol works effectively with plasma obtained...
28 July 2024 3,559 0 View
I have been looking for the principle of Cysteine-carbazole-sulfuric acid reaction in research papers and books, but couldn't find it. This reaction is mainly used for the quantification of...
23 July 2024 3,891 0 View
Sulfuric acid has changed color to yellow.
16 July 2024 6,964 0 View
Hey! I aim to generate a transgenic knockin zebrafish line that mimetizes a genetic condtition that leads to a certain disease on human. To do so, I need to insert a codon for mutagenic aminoacid...
14 July 2024 6,240 0 View
Hi, Attempted to extract cfDNA from a 4 mL plasma sample, with poor yield. Went through the manual in the section regarding poor yield, and feel that this does not apply. Some potential...
14 July 2024 7,475 1 View
Studying a specific monogenetic disease based on mutations in different individual genes. I try to recreate the disease phenotypes of donors in vitro. I knocked out a gene x with above 90%...
12 July 2024 6,313 0 View