What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

How much cell pressure and back pressure should I apply to start for triaxial test for different types of soil?

We have recently purchased a triaxial test instrument (MATEST) for testing different types of soil. After installing software, we are facing a issue of saturation where the samples does not...

23 February 2021 7,194 4 View

Why FTIR can not detect bases of nucleic acid in extracellular polymeric substances (EPS)?

I characterized functional groups of EPS using FTIR. Goups of sugars and proteins (e.g., -OH, amide, -COOH) as well as phosphate group of nucleic acid were readily detected. However, peaks of...

05 February 2021 4,405 1 View

Spatial thinking and geographical reasoning: what are their conceptual differences and interrelations in teaching geography?

I am conducting a state-of-the-art analysis of these concepts. If you have contributions, please, send me.

17 January 2021 1,123 3 View

Why my BOC-protected compounds got deprotected during evaporation?

I purified my Boc-protected molecule using HPLC. The eluent is ACN/H2O/(0.05% TFA) . After I evaporated the solvent (65 oC), the product was collect as the MS result showed ~20% of the product was...

14 January 2021 5,487 5 View

Who can tell me the easily acquired Pb hyperaccumulator?

The common used Pb hyperaccumulators in the experiment, which and can buy from the business. Thank you!

14 January 2021 460 3 View

Does anybody have the recipe for making a home made R electroporation buffer for Neon transfection systems?

I tried the recipes below in two cells lines: 1.DPBS 2.DPBS+MgCl2(1mM) 3.DPBS+Sucrose(250mM) 4.DPBS+MgCl2(1mM)+Sucrose(250mM) 5.R Buffer (Neon) the results showed that R buffer is the best one in...

06 January 2021 6,776 1 View

Why there is need to calculate the chlorophyll content and chlorophyll index. What protocol should I used for calculating chlorphyll in mustard?

I want to know the best and effective method/ protocol to calculate the chlorophyll from mustard plants. Please suggest some relevant and the methods or protocols from which I can easily calculate...

05 January 2021 9,882 3 View

How can I downgrade Networkx 2.5 to 2.3 version?

When I run the command according to the GROMACS tutorial, I got the below result This script has been tested with NetworkX 2.3, and 2.4 is buggy. Please install version 2.3 for best...

27 December 2020 4,699 3 View

Are there any way to make the comparison between different analysis of reliability possible?

10 December 2020 7,922 4 View

Can anyone suggest me the best way for Regression analysis of Accidents?

Dear Altruist, I want to make a regression analysis based on accident data. But I am not finding the necessary information about what type of regression is more applicable for analysis accident...

23 February 2021 9,956 3 View

(western blot) can methanol damage my antibody signal?

I accidentally rinsed with methanol after adding my primary antibody to my membrane. I noticed after ~10 seconds and immediately replaced it with PBST (3x15 min washes). Will my antibody signal be...

12 February 2021 5,824 3 View

What is the reference value of THD in BLDC MOTOR speed control ?

i used to control EHO+ANFIS to MRPID to Landsman converter for bldc drive i got 7% but some of them are telling IEEE standards are < 5% is it correct for Drives ?

07 February 2021 5,892 1 View

Can't run my IPMSM drive system with DC-supply higher than 125 V. Have anyone had similar problem?

Hey, I try to run my 4 pole IPMSM with PWSVM and use a typical controller with PI-controller with a current loop and speed loop. When I run my system with DC-supply 100 V with a steady-state speed...

28 January 2021 7,354 8 View

How to build a Jupyter Notebook page to access remote Molecular Dynamics results?

Hi all, I am hoping for some assistance. I (the researcher) would like to know whether it is possible and how to go about building a Jupyter Notebook that a collaborator could load in their web...

26 January 2021 1,027 1 View

I accidentally poked my finger with very thiny tweezers after I used them to remove a slide from A549 cell culture??

I accidentally poked my finger with very thiny tweezers after I used them to remove a slide from A549 cell culture. I got poked after sprayed them with 70 % alcohol and dried them right away. Is...

07 January 2021 9,412 2 View

What materials besides gold can be infused into window panes?

Hello, Driving around, I have noticed a lot of office buildings where the glass panes have been infused with gold. The thin layer of gold reflects the sun and keeps the office at a comfortable,...

06 January 2021 976 4 View

When we regulate by tricolor light signals in VISSIM, should we make all the conflict zones inactive?

hi everyone, I have developed a simulation of a roundabout with a fixed time regulation, the lines of traffic lights in the roundabout are for vehicle storage. my question is: when we regulate by...

16 December 2020 1,923 3 View

Need help in vissim VisVap ? pedCrossing ?

Hello everyone, hope you are doing well. I made a simple traffic actuated control logic using VisVAP Stage 1 is for Vehicle Stage 2 is for Ped the compilation in VisVap run with success, but...

07 December 2020 553 3 View

I messed up the DMSO and serial dilution standards in my MSD assay...How can I save my data?

MSD requires 8 standards that are serially diluted down in order to produce a standard curve. In the final row of my MSD Assay, I accidentally pipetted DMSO (same volume) into the last 4 wells of...

24 November 2020 3,376 2 View