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Matrigel is very excellent material for tumor engraftments. We know its positive effects only. Does anyone know about its immunological effect on mouse immune system ? Thanks and regards Anuj
03 April 2019 5,380 0 View
Hi, I am working with DBA/2 mice and I want to know spleen weight of naive and nulliparour 10-12 week old female DBA/ mice Please help me Regards Anuj
10 November 2017 1,166 0 View
I have 4T1 cell line and it is contaminated with Mycoplasma .I passed it through nude mice subcutaneous injection and cultutered tumor cell and tested for mycoplasma presence and found it's...
10 November 2016 10,008 6 View
I want to develop LTPA cell line tumor model in mouse and tried Balb/c and C57BL/6 mice but I did not get any tumor If anyone have any idea please help me to get the model Regards Anuj
10 November 2016 8,857 0 View
If anyone have any adea about the corelatiob between mycoplasma and murine tumor cell line like B16F10, CT26 and 4T1 Anuj
07 August 2016 1,079 7 View
Please provide your inputs on this topic
07 August 2016 1,088 0 View
Currently I am dealing with Calu-6 cell line . I want to keep the cells in T225cm2 flask with 2.8 million/T225cm2 with medium volume of 20mL/flask. I want to know what will be the rate of Gaseous...
04 May 2016 7,527 7 View
Recently I injected a cell line in female nude mice and after 7-8 days of injection , I found redness on skin of most of the mouse and after redness, whole skin of the animal have covered with...
31 December 2015 7,852 1 View
I am trying to harvest cell from matrigel coated flask plaese guide me I have tried trypsin but didn't work . I don't have matrigel dissociation medium
07 August 2015 6,448 5 View
Thanks in advance for your replies.
04 May 2015 4,362 4 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...
03 August 2024 3,851 1 View
what are the top 3 challenges to the advancement of the field of Radiogenomics in cancer research? is it the availability of easily available low-cost matched imaging and biosamples with clinical...
03 August 2024 5,828 4 View
