Im initiating a cell suspension culture from callus of Lupinus mutabilis, the calli I have are friable but once I pass them to a liquid medium they turn brown and compact. I´ve tried adding PVP and celulase but the difference is unnoticable.
Hi Paula, you can play with several factors inorder to reduce the browning. one of such factor is ammonium nitrate ( besides that ammonium is toxic to the invitro growing cells). activated charcol is another potencal alternative to reduce the browning.
good luck for your work
Hi Paula,
Please also see this discussion (on RG) to see how to reduce browning in tissue culture:
https://www.researchgate.net/post/How_do_I_reduce_the_Browning_in_Tissue_culture_media
Hi Paula
May be you can try with some anti browning agent such as Ascorbic Acid. May be it can help you
Good Luck
Why used liquid medium , you can used semi solid media if you see that liquid media gave you brown and compact callus, Yet, you can addition activated charcoal to liquid media , The hormonal balance in the nutrient medium 'reduces this phenomenon.
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