S. V. G. Nirosha Priyadarshani

2 Questions 8 Answers 0 Followers

Questions related from S. V. G. Nirosha Priyadarshani

I need to stain protoplast with Rhodamine B. Pls advice me to prepare Rhodamine for protoplast staining and observation?

How to prepare Rhodamine B for staining protoplast? How to stain? What is the excitation level i should use to observe stained protoplast?

28 November 2017 2,911 3 View

Please can someone suggest me a methodology for protoplast isolation from pineapple leaves?

I have tried protoplast isolation from pineapple leaves several times using 0.25% macerozyme and 1%cellulase R-10. 0.4M mannitol was also used. But almost all protoplasts were damaged. What is the...

24 April 2017 9,435 2 View