Dataset: Orbitrap velos CID MS/MS spectra ?

Hi, I want the MS2-CID spectra from orbitrap velos mass spectrometry of organism: human (Mod: C+57) for testing my peptide identification algorithm developed. Large set of orbitrap velos dataset...

16 November 2020 8,261 1 View

Why nodal rotational velocities are zero in *STEADY STATE TRANSPORT?

I am performing a Steady State rolling analysis of a tire using input files. I have applied rotational velocity to all the nodes in the tire by creating a set using *TRANSPORT VELOCITY. I want to...

27 July 2020 8,873 2 View

The resistance of the sensor was decreased after doping at room temperature why?

the resistance of the sensor was decreased after doping at room temperature why

18 June 2020 5,636 2 View

How to develop a ordinal scale? What psychometric properties must be assessed?

I am planning to develop a ordinal scale to assess a motor function like sitting to standing. I like to know method to develop and test its relevant psychometric properties. I will be happy if...

17 July 2019 8,857 4 View

What are some sources for hypothesis in qualitative research?

I have been reading on hypothesis in qualitative research. I find convincing suggestions. most do not cite their sources.

06 May 2019 3,736 4 View

Is there any open source tool to identify peptide from peak list input file using Sequest & Mascot algorithm?

I have an input file containing precursor mass and peak list (m/z and Intensity) of tandem mass spectrum. The file contains multiple spectrum data. I need to identify peptide using sequest and...

09 April 2019 8,354 4 View

Saltspray life of brass plating ( Steel) sample ?

We are doing brass plating for chain plates , Saltspray life to be finalized , What is actual salt spray life of Nickel & Brass top coat .

31 March 2019 2,720 1 View

What are the chemical agents or compounds which designs the nanostructures?

I like to synthesis a nanomaterial,but I have a doubt on choosing the precursor for to get a desired nano structure?

17 October 2018 5,959 3 View

Why BxPC3 pancreatic cancer cells die due to Akt plasmid transfection with Lipofectamine 2000? There is approxiamtely 60%-70% cell death?

14 August 2018 2,624 3 View

How to grid downscale CMIP5 climate data grid to 0.5 degree using Matlab ?

08 July 2018 2,235 2 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View