How should I prepare solutions of divalent metals at neutral pH?

Hello, I would like to prepare metal solutions of 50 mM Mg, Zn, Ca, and Cu (separately) from their chloride salts, to study protein-metal-ion interaction. For this reason, I would prefer that it...

25 November 2020 1,924 11 View

What is the limit of detection for Pour Plate method?

i preformed several test using the pour plate method with no dilution, i counted 0-4 colonies per plate, inoculum 1 ml, agar-15ml, which meen 0-400cfu/100ml. my goal is to achive

26 June 2019 7,406 2 View

How to calculate the specific capacitance of graphene foil using galvanostatic charge-discharge?

The proper way is to normalize it according to the weight of the electrode in order to get the specific capacitance in F/g units? or should I normalize it according to the geometric area to get it...

28 January 2019 4,796 3 View

Does anyone know if GlycoBlue affect cells in vitro ?

I would like to transfect RNA into cells and I was wondering if it is going to be toxic ?

07 August 2017 4,909 3 View

Does anyone know the material E40CDV12?

09 April 2016 2,738 3 View

Should there be a correlation between band size on gel and SYBR green signal in the real time PCR run?

19 January 2016 4,703 3 View

Is there logic behind using Annexin V FACS with another antibody?

02 December 2015 605 3 View

High miniprep yield followed by extremely low Ct values?

I have been using Clone jet PCR cloning kit with a pJET1.2 blunt as a vector. The insert is from a clean PCR product that went through a blunting reaction (supplied with the cloning kit). The E....

19 October 2015 8,587 6 View

How do I use two way ANOVA on real time PCR results?

09 March 2015 8,424 6 View

I see primer-dimers only in my qRT-PCR negative controls. Does it mean everything is OK?

Hello, I have just started with some qRT-PCR for my reasearch and Im doing a calibration of my reactions. With all the primers I checked(5 genes) I get an amplification of my NTCs and NRTs, in a...

06 January 2015 5,961 8 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

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Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View