Ori Elad

11 Questions 15 Answers 0 Followers

Questions related from Ori Elad

Should there be a correlation between band size on gel and SYBR green signal in the real time PCR run?

Hello all. I have been working with a sequence that is a 571 bp clone, and I have a primer pair that gives a 150 bp amplicon. When I ran the system in real time for a calibration curve I got some...

20 January 2016 4,866 3 View

Is there logic behind using Annexin V FACS with another antibody?

Good evening. I am wondering if it is possible to do an Annexin V FACS staining on B cells extracted from the bursa together with staining with an antibody against surface IgM. I read that B...

03 December 2015 800 3 View

High miniprep yield followed by extremely low Ct values?

I have been using Clone jet PCR cloning kit with a pJET1.2 blunt as a vector. The insert is from a clean PCR product that went through a blunting reaction (supplied with the cloning kit). The E....

20 October 2015 8,770 6 View

Are IP3 levels in B cells indicative of activation through BCR recognition?

I am looking into ways to examine B cell activation after antigen recognition through the B cell receptor. Going through literature, I saw that antigen recognition in this pathway would increase...

10 September 2015 7,284 2 View

How do I use two way ANOVA on real time PCR results?

Hello all. I want to test whether two different factors - concentration (3 treatments) and incubation time (2 treatments) have an effect on gene expression. I ran the gene, and I have the Ct...

10 March 2015 8,651 6 View

How do I calculate standard errors for normalized log2 converted gene expression data?

I ran an experiment with a ref gene (3 biological repeats x 3 technical repeats) and normalized gene expression of my target genes to the ref gene. I decided to present the data as log2 fold...

06 January 2015 6,396 6 View

Has anyone had any experience with storing cell culture slides for a TUNEL assay?

Hello all. I have read a protocol stating that post paraformaldehyde fixation (4% in PBS) cell culture slides can be preserved in 70% ethanol at -20 deg. cel. until use (not good for long term,...

23 December 2014 4,051 2 View

Can anyone recommend a process for preparing and storing slides for a TUNEL assay?

I want to prepare slides of DT-40 cells for a TUNEL assay. My question is, is there a protocol for preparing them and fixing them so they can be stored at -80 degrees for about a week?

30 September 2014 9,118 2 View

Does anyone have a specific recommendation for a DNA pol or mix fit for PCR ?

I'm using bisulfite treated DNA as template for sequencing purposes.

08 September 2014 8,541 6 View

Any problems using the same primer set twice?

Theoretically, is there a problem with running a PCR and then running the same system again, with the same primers, with the product of the first reaction to further amplify the product? The...

21 August 2014 3,941 8 View

Can I use the Miniprep product to transform a new batch of E. coli?

My culture with positive bacteria in it is unusable, and the plates with positive insert bacteria (E. coli) are stored @ 4 deg. cel. and I can't get them to regrow in an overnight culture. I do,...

29 June 2014 2,328 6 View