What is the limit of detection for Pour Plate method?

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SW CNT INDUSTRIAL PRODUCTION - turnkey pilot lines ?

Hi any suggestion to get in touch with teams able to design build and test industrial lines for single wall CNT production? best OTTO

24 February 2023 1,982 0 View

How should I prepare solutions of divalent metals at neutral pH?

Hello, I would like to prepare metal solutions of 50 mM Mg, Zn, Ca, and Cu (separately) from their chloride salts, to study protein-metal-ion interaction. For this reason, I would prefer that it...

25 November 2020 2,107 11 View

How to calculate the specific capacitance of graphene foil using galvanostatic charge-discharge?

The proper way is to normalize it according to the weight of the electrode in order to get the specific capacitance in F/g units? or should I normalize it according to the geometric area to get it...

28 January 2019 4,990 3 View

Does anyone know if GlycoBlue affect cells in vitro ?

I would like to transfect RNA into cells and I was wondering if it is going to be toxic ?

07 August 2017 5,037 3 View

Is there any work analyzing liberty in terms of form and content?

I’m looking for any work done on the concept of freedom (interchangeable with liberty) which analyzes it in terms of form and content. My meaning is that the concept of freedom is construed as...

17 November 2016 4,720 9 View

Are there any interpretations of Rousseau's concept of freedom in analytical contexts, particularly in light of the negative/positive controversy?

I'm looking for any work dealing with Rousseau in relation to Berlin's 'Two concepts of liberty' and the debates which follow, or to contemporary work on freedom such as Raz' autonomy based...

03 July 2016 2,972 4 View

Should there be a correlation between band size on gel and SYBR green signal in the real time PCR run?

Hello all. I have been working with a sequence that is a 571 bp clone, and I have a primer pair that gives a 150 bp amplicon. When I ran the system in real time for a calibration curve I got some...

19 January 2016 4,840 3 View

Inconsistent results while using Weka classifiers who were read from disk – any suggestion why?

Hi, We’re experiencing strange behavior while using Weka Java implementation classifiers (say J48 for example). We build a classifier Test it on examples, getting results A Saving the classifier...

09 December 2015 2,913 2 View

Is there logic behind using Annexin V FACS with another antibody?

Good evening. I am wondering if it is possible to do an Annexin V FACS staining on B cells extracted from the bursa together with staining with an antibody against surface IgM. I read that B...

02 December 2015 756 3 View

High miniprep yield followed by extremely low Ct values?

I have been using Clone jet PCR cloning kit with a pJET1.2 blunt as a vector. The insert is from a clean PCR product that went through a blunting reaction (supplied with the cloning kit). The E....

19 October 2015 8,738 6 View

Is changing the medium during the experiment overestimate the results?

I am experimenting with cancer and non-cancer cells in a 12-well plate for 4 days with a seeding density 1*10^4/well, however, I noticed that the control group growth rate slows down on D3. Should...

07 August 2024 2,283 2 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Absorption coefficient of methane?

Hello, Can anyone provide me with the absorption coefficient of methane gas at 7.7 um? Any reference?

06 August 2024 980 5 View

Can you recommend a 96-well plate for minimal cell adherence to the wells during phagocytosis experiments?

I have been performing short phagocytosis experiments using 96-round bottom, tissue culture treated, well plates (Falcon, 353077). I culture the cells with the phagocytic cargo for 2-4hrs, wash...

04 August 2024 5,228 4 View

PBMC infection with virus?

In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...

01 August 2024 4,403 4 View

Found this object in the cell culture - do you have any suggestions what it might be?

Dear researchers, Today, we found this object in one of the wells of a 96wp culture plate. The plate contained MEC-1 cells and GelTrex. I am quite curious what this is (because it's not cells). Do...

01 August 2024 2,250 2 View

Some new emerging problems on application of RL for scheduling in IoT networks?

I have seen plenty of existing works on applied Reinforcement Learning (RL) policies for optimized scheduling in IoT networks including Q-learning, DQNs, and the newer ones including PPO for...

01 August 2024 8,754 2 View

Publishing a checklist?

Does anybody know of entomological Journals without page charges that would publish an extensive checklist (Hong Kong Aculeates wasps) covering 350 species (of which more than half are new to Hong...

01 August 2024 6,134 2 View

What are the roles of innovation in achieving the Sustainable Development Goals (SDG)?

31 July 2024 3,533 2 View

Hi there, someone has the SeinFit software for windows because I cannot download it?

DOS version.

29 July 2024 6,064 1 View