How should I prepare solutions of divalent metals at neutral pH?

Hello, I would like to prepare metal solutions of 50 mM Mg, Zn, Ca, and Cu (separately) from their chloride salts, to study protein-metal-ion interaction. For this reason, I would prefer that it...

25 November 2020 1,924 11 View

What is the limit of detection for Pour Plate method?

i preformed several test using the pour plate method with no dilution, i counted 0-4 colonies per plate, inoculum 1 ml, agar-15ml, which meen 0-400cfu/100ml. my goal is to achive

26 June 2019 7,406 2 View

How to calculate the specific capacitance of graphene foil using galvanostatic charge-discharge?

The proper way is to normalize it according to the weight of the electrode in order to get the specific capacitance in F/g units? or should I normalize it according to the geometric area to get it...

28 January 2019 4,796 3 View

Does anyone know if GlycoBlue affect cells in vitro ?

I would like to transfect RNA into cells and I was wondering if it is going to be toxic ?

07 August 2017 4,909 3 View

Should there be a correlation between band size on gel and SYBR green signal in the real time PCR run?

19 January 2016 4,703 3 View

Is there logic behind using Annexin V FACS with another antibody?

02 December 2015 605 3 View

High miniprep yield followed by extremely low Ct values?

I have been using Clone jet PCR cloning kit with a pJET1.2 blunt as a vector. The insert is from a clean PCR product that went through a blunting reaction (supplied with the cloning kit). The E....

19 October 2015 8,587 6 View

How do I use two way ANOVA on real time PCR results?

09 March 2015 8,424 6 View

How do I calculate standard errors for normalized log2 converted gene expression data?

I ran an experiment with a ref gene (3 biological repeats x 3 technical repeats) and normalized gene expression of my target genes to the ref gene. I decided to present the data as log2 fold...

05 January 2015 6,166 6 View

Has anyone had any experience with storing cell culture slides for a TUNEL assay?

22 December 2014 3,895 2 View

How to quantify carbon deposition on CuZnAl catalyst?

Dear All, I want to quantify carbon deposition on the surface of the CuZnAl catalyst. I have tried thermogravimetric analysis, but I've got weight gain as the active Cu- get oxidised. Does anyone...

01 March 2021 1,907 3 View

Why Trivalent chromium start precipitating above PH 5 ? Moreover discuss difference between adsorption of chromium and precipitation of chromium ?

I am a student of MS Environmental Engineering and started my research phase. I am working on metal removal ( chromium iii ) by using activated carbon prepared from the agricultural feedstock. So...

01 March 2021 5,475 2 View

Is there any research regarding how training and development is going to change in the era of Fourth Industrial Revolution?

As the organizations getting prepared for the fourth industrial revolution, to enhance the skills of their workforce what type of changes are necessary and going to occur in the training and...

01 March 2021 3,199 3 View

How can I assign a velocity along one direction to CO2 molecules in a molecular dynamics simulation using GROMACS?

Dear all, I am trying to perform a MD simulation about the interaction between CO2 and a polymer. I create my model, solvate it and perform the NVT and NPT equilibration. Now I am not able to...

26 February 2021 10,014 3 View

Can anyone share his experience regards Smoothing in origin?

I am working on TiO2 composite material and the results of XRD have a lot of small/junk peaks and while I apply a smooth filter in Origin then the other characteristics peaks disappear so anyone...

26 February 2021 8,183 3 View

CGA 300 Regulator for CO2 cylinder?

Has anyone ever tried to deliver CO2 with a CGA 300 regulator? I know that a CGA 320 regulator is standard for CO2. The issue is that I currently do not have any free CGA 320 regulators. I also...

25 February 2021 3,292 3 View

What are the methods used for CNT..?

Respected Professor's i hope you are doing well and happy. i need some information about carbon Nanotubes. before blending the CNT with the matrix the CNT has been introducing in some treatments....

24 February 2021 5,383 5 View

How to clean up TEM grid with plasma cleaner?

I need to clean the hydrocarbon contamination on the carbon substrate of TEM grid. However, I heard that substrate could be broken by the powerful plasma. My substrate is pure carbon, with ~3nm,...

23 February 2021 3,262 5 View

What are some underexplored theories and concepts in Activated Carbon as A Water Filter?

Hi, me and my team mates are currently planning to pursue the topic of Activated Carbon from Coconut as a Water Filter. And we would like to ask for your suggestions and knowledge on what specific...

22 February 2021 6,569 3 View

What are some underexplored theories and concepts in Activated Carbon as A Water Filter?

Hi, me and my team mates are currently planning to pursue the topic of Activated Carbon from Coconut as a Water Filter. And we would like to ask for your suggestions and knowledge on what specific...

21 February 2021 7,561 4 View