My experiments include ssDNA, dsDNA, and DNA-PNA hybrids
I encountered the software while searching for computerised morphometric tools based on images. The software is easy to install and the article published on its making is quiet thorough, but I...
11 December 2022 7,304 2 View
I have seen this phenomenon. Qpcr product band size was validated by Gel electrophoresis. Melting peak tempreture of my product is 79°C.
30 September 2020 529 3 View
Recently, I have been investigating of expression of Sall-4 in two different types cancers. Due to of acceptable melting peak of Beta Actin(and Cq=17), I am sure the quality of cDNA is pretty...
08 August 2020 3,175 8 View
Anyone holds information regarding the typical hourly distribution of rain events in Australian cities (i.e. rain intensities, hour by hour) is welcome to contact me for a shared...
31 October 2016 5,164 11 View
I'm not quietly sure if it 40 or 4O. I would like the equivalent name in AISI. I think it is a French name.
09 April 2016 2,902 3 View
Hello all. I have been working with a sequence that is a 571 bp clone, and I have a primer pair that gives a 150 bp amplicon. When I ran the system in real time for a calibration curve I got some...
19 January 2016 4,840 3 View
Good evening. I am wondering if it is possible to do an Annexin V FACS staining on B cells extracted from the bursa together with staining with an antibody against surface IgM. I read that B...
02 December 2015 756 3 View
I have been using Clone jet PCR cloning kit with a pJET1.2 blunt as a vector. The insert is from a clean PCR product that went through a blunting reaction (supplied with the cloning kit). The E....
19 October 2015 8,738 6 View
Hello all. I want to test whether two different factors - concentration (3 treatments) and incubation time (2 treatments) have an effect on gene expression. I ran the gene, and I have the Ct...
09 March 2015 8,626 6 View
Hello, I'm researching gene expression of cells after some treatment, which cause apoptosis. when I compare 2 different population of cells (WT and crispr KO of some gene), I get the same Ct for...
03 February 2015 3,613 7 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.
07 August 2024 8,782 3 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
Dear readers, Thanks for your attention. I am wondering about the health economic problem of quantifying the value of interventions which a) prevent, b) improve symptom profile and c) ultimately...
05 August 2024 3,246 1 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
I am attempting to conjugate PEG to an amino acid at the C-terminus, for the purposes of producing nanoparticles. I have been told that PEG modified with amine groups can be used for this purpose,...
31 July 2024 2,033 1 View
What information we can get from PXRD analysis other than from SCXRD analysis of a crystal ?
30 July 2024 6,261 4 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
