How to validate image labeling quality within dataset?

Hello, it is about creating a dataset for semantic segmentation with three classes. The problem is that one class dominates with >90% and one class is

18 February 2021 4,254 5 View

Are there any DNA extraction kit from resins?

Hi everyone, I'm looking for a DNA extraction kit or some protocols to obtain the total DNA from resin samples. For this purpose, some studies suggest the use of plant extraction protocol of...

25 October 2020 3,898 3 View

How to calculate the effect size of a mixed models anova (from existing research papers)?

Hi all I'm comparing some research papers for my university. The most studies use a mixed model ANOVA design, but did not specify the effect sizes. 1. Is it possible to (re)calculate the effects...

23 October 2020 5,013 3 View

Antibody : loss of binding at high concentration (beyond saturation) Why?

Hi everyone, It is commonly observed that some antibodies presents a binding saturation curve that goes down at high concentration (at concentration beyond saturation). Does anybody have a good...

20 August 2020 778 5 View

TRP1 deficient yeast strain not growing on 5FAA containing medium?

05 August 2020 3,010 5 View

What would be the ideal number of epoch for my Neural Network?

Hello, If I am training my Neural Network with training data comprised of 908 batches, as the rule of thumb, would 20 epochs for training be sufficient? If 20 epochs are not sufficient, does...

26 March 2020 2,291 6 View

How important is it to set seed in experiments in computer science?

Hello, If I am going to submit my work to a CS conference, and also make my Python code available publicly, is it absolutely necessary that I set seed in my Python code? I ran an experiment, but...

07 March 2020 1,832 3 View

How to process ARC dataset with GTP2 Double Heads Model?

Hello, I am interested in processing the ARC dataset (http://nlpprogress.com/english/question_answering.html) with the GPT2 double heads model neural network. The dataset (tab delimited) is...

22 November 2019 3,796 1 View

How to distinguish "elementary English" from "advanced English"?

Hello, When we take a look at English test prep books that are specially designed for ESL learners, we often see in their title that the book is for "learners at elementary level", "learners at...

15 November 2019 5,393 1 View

Can someone please guide me to a software that I can use to create time lapse models for the marine environment?

I am working on a concept related to the formation of coral reefs and I need to demonstrate the concept in the form of a visual model that changes over time. I am fairly new to this area of...

16 November 2018 9,276 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View