How to validate image labeling quality within dataset?

Hello, it is about creating a dataset for semantic segmentation with three classes. The problem is that one class dominates with >90% and one class is

18 February 2021 4,254 5 View

Are there any DNA extraction kit from resins?

Hi everyone, I'm looking for a DNA extraction kit or some protocols to obtain the total DNA from resin samples. For this purpose, some studies suggest the use of plant extraction protocol of...

25 October 2020 3,898 3 View

How to calculate the effect size of a mixed models anova (from existing research papers)?

Hi all I'm comparing some research papers for my university. The most studies use a mixed model ANOVA design, but did not specify the effect sizes. 1. Is it possible to (re)calculate the effects...

23 October 2020 5,013 3 View

Antibody : loss of binding at high concentration (beyond saturation) Why?

Hi everyone, It is commonly observed that some antibodies presents a binding saturation curve that goes down at high concentration (at concentration beyond saturation). Does anybody have a good...

20 August 2020 778 5 View

TRP1 deficient yeast strain not growing on 5FAA containing medium?

05 August 2020 3,010 5 View

What would be the ideal number of epoch for my Neural Network?

Hello, If I am training my Neural Network with training data comprised of 908 batches, as the rule of thumb, would 20 epochs for training be sufficient? If 20 epochs are not sufficient, does...

26 March 2020 2,291 6 View

How important is it to set seed in experiments in computer science?

Hello, If I am going to submit my work to a CS conference, and also make my Python code available publicly, is it absolutely necessary that I set seed in my Python code? I ran an experiment, but...

07 March 2020 1,832 3 View

How to process ARC dataset with GTP2 Double Heads Model?

Hello, I am interested in processing the ARC dataset (http://nlpprogress.com/english/question_answering.html) with the GPT2 double heads model neural network. The dataset (tab delimited) is...

22 November 2019 3,796 1 View

How to distinguish "elementary English" from "advanced English"?

Hello, When we take a look at English test prep books that are specially designed for ESL learners, we often see in their title that the book is for "learners at elementary level", "learners at...

15 November 2019 5,393 1 View

Can someone please guide me to a software that I can use to create time lapse models for the marine environment?

I am working on a concept related to the formation of coral reefs and I need to demonstrate the concept in the form of a visual model that changes over time. I am fairly new to this area of...

16 November 2018 9,276 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Do you know good and user-friendly programs to measure plant quantitative traits?

For my research i will need to measure plant quantitative traits (especially leaves area and roots length, but would be nice to add some more information). I recently discovered...

01 March 2021 5,035 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View