14 June 2024 1 4K Report

Hello, could you please give me precise details of the antibodies that can be used to perform quantitative immunofluorescence of AT1Rs in the mouse brain?

More Dominique Hyacinthe Hatho Towo's questions See All
Do cancer cells behave differently to healthy cells when exposed to hyperthermia ?

Dear colleagues, I'd like to find some references documenting the biological reactions of tumour versus normal tissue to heat-based ablations, in terms of cellular sensitivity/resistance and...

13 March 2024 4,814 2 View

(LCA - Ecoinvent) Where can I find the hypotheses behind the Ecoinvent EF 3.1 Caracterisation factors ?

Hello, I study the impact of activities on Oceans. 1. Ecoinvent is mistakingly considering the "infinite dilution" hypothese, forgetting that the dilution of pollution is usually done through its...

07 December 2023 8,172 1 View

Whistling 10 years ago: publication of the following was refused! a shame?

While «hair growth» is an extremely popular item. On February 12, 2013, there were 61,600,000 hits via Google and there were 70,400,000 hits on July 11, 2013? The interest grows on the web as the...

09 November 2023 9,327 1 View

Which tissue is closest to the prostate gland in terms of MRI features (e.g., relaxation times) and physiological characteristics ?

As the pig prostate (our animal model) is relatively difficult to access for an ablation procedure, we'd like to use a proxy organ.

03 September 2023 4,822 0 View

What is the best liquid-liquid extraction method to separate fatty alcohols for LCMS testing with or without derivatizing?

I have been trying to isolate a hydrophobic fatty acid compound from an aqueous extract with a surfactant. The fatty acid compound is Insoluble in water , soluble in ether, chloroform,...

30 August 2023 6,719 0 View

How to do an MRI image average and registration?

Hello, We have done an MRI acquisition of an animal brain using three times the same T1 sequence. However, we are now trying to average these three data sets and do a 3D reconstruction. This is...

22 March 2023 6,565 1 View

Why Rhodomonas salina does actively build aggregates?

Hi all, I recently observed that a Rhodomonas salina strain builds aggregates after only a few minutes. Does anyone have any idea why this is the case? Attached are two jpgs. Sorry for the poor...

22 February 2023 3,089 0 View

Behaviour Tests - Colour discrimination in birds of prey?

Dear Community, I’m searching for a researcher / research group who is working on behavioural tests – colour discrimination in birds of prey. I would be very grateful for advice and hints! Best,...

24 November 2022 9,176 1 View

Bird-window collision research in France?

Dear Community, I'm searching for bird-window collision research in France. Maybe somebody is aware of a research group which is looking into this topic? Best, Dominique

02 August 2022 9,288 2 View

How can I eliminate copepod contamination in Ostrea edulis larvae samples?

Dear reserachers, currently I am working with Ostrea edulis larvae. However, I can't get rid of copepods via mechanical separation as the have more or less the same size as the oyster larvae. I...

29 June 2022 7,335 2 View

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Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

ANY free software for reconstructing neurons in the microscopic image?

Hi everyone, I am working on brain slices for visualizing a protein in the soma and dendrites, using a fluorescence tag. However, I need a tool (not paid) for reconstruction of the whole neuron,...

04 August 2024 4,725 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

In what part of the brain´s rabbit bdnf is located and include references please?

I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....

04 August 2024 762 1 View

Do You Know Untreated TMJ Affects Your Brain And Entire Body?

Do You Know Untreated TMJ Affects Your Brain And Entire Body?

04 August 2024 6,282 1 View

What is the best protocol for freezing the rat brain in preparation for protein detection by Elisa ?

What is the best way to freeze the rat brain - liquid nitrogen, Dry ice , isopentane - after isolation for later protein investigation by Elisa?

01 August 2024 1,722 1 View

How to Post-fix Mice pup's brains?

I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...

31 July 2024 1,123 4 View

How to make a Lateral flow control line more uniform?

We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...

31 July 2024 4,510 3 View

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