Jitendra Kumar Shandilya

7 Questions 312 Answers 0 Followers

Questions related from Jitendra Kumar Shandilya

How to Interprete Phagocytosis Assay by flowcytometry?

I am using "pHrodo™ Red E. coli BioParticles™ Phagocytosis Kit for Flow Cytometry" to access phagocytosis in neutrophils on perepheral whole blood. As per the protocol i followed all the...

14 December 2020 8,825 0 View

Alternative method of Permeablization in IL17A assay by flowcytometry?

I am evaluating Th17 cells by staining stimulated cells with IL17A and IFN-Y antibody by flow cytometry. Permeabilization step was done with Cytoperm/cytofix reagents (BD Bisciences) yet and the...

02 December 2020 4,814 1 View

NK cells markers; is cd56 alone enough or CD16/CD56 ?

We read that NK cells are CD 3- and CD 56+/CD 16+. Recently I and across two IVD approved kits from two manufacturer for lymphocyte subset assay. Kit from BD (cat no. 644611) have CD16/CD56+...

04 August 2020 9,342 5 View

Differentiation of platelets and RBC in flowcytometry?

I want to evaluate platelet marker. While running whole blood (10uL), stained antibody on flowcytometer on log scale o Forward scatter and side scatter, find a "L" shape population. I am not able...

22 July 2020 6,020 2 View

Am i allowed to increase gain of fluorescence channel in Beckman Coulter Navios Flowcytometer?

We have a Beckman Coulter flowcytometer (2 Laser, 8 parameters). Gain of the FSC adn SSC is adjusted accordingly to separate two merging populations. There is option to select different predefined...

01 January 1970 1,577 1 View

How does RBC lysis buffer lyse only RBC and no other cells?

While performing staining for flowcytometry we need to lyse RBC. We usually use BD 1X RBC lysis buffer for 10-15 minutes. My question are; 1. Does that buffer lyse only RBC not other cells? 2....

01 January 1970 9,110 4 View

What would be the fate of PBMCs in culture?

What would happen to PMBCs When we isolate PBMCs from blood from a healthy person, and culture them in complete medium (RPMI1640+10%FBS+PanStrap) without any stimulation? My questions are; 1....

01 January 1970 1,843 5 View