I want to know whether cancer cells like MCF-7, MBA-MB 231, Colo etc. secrete ECM when cultured in monolayer? If yes, what is molecular composition of such matrix? Any reference literature available on this?
Yes, cancer cells when cultured as monolayers secrete thei own ECM components. We have worked on this subject some years ago with glioma cells (see the attached article). Each cell lines will secrete its own ECM component profile. Cancer cells cultured as monolayers secrete these ECM components to reinforce their attachment on the bottom of the flask and avoid anoikis.
Happy New Year 2016!
Robert
Yes, cancer cells when cultured as monolayers secrete thei own ECM components. We have worked on this subject some years ago with glioma cells (see the attached article). Each cell lines will secrete its own ECM component profile. Cancer cells cultured as monolayers secrete these ECM components to reinforce their attachment on the bottom of the flask and avoid anoikis.
Happy New Year 2016!
Robert
Hi Pooja Patheja, the composition of extracellular matrix (ECM) produced largely depends on the particular cell type, while the actual matrix deposition in the culture dish is also strongly influenceed by cell shape, degree of cell polarity as well as tumor cell properties. By the way, the forementioned PLoS One paper describes growth of MCF-7 cells on a ECM scaffold prepared from an experimental tumor which presumably shares properties with Matrigel. The latter is isolated from a special transplantable mouse tumor, containing huge amounts of basement membrane/ BM components. Like other epithelial cells (e.g. epidermal cells or BHK) MCF-7 (more 'normal') and MBA-MB231 should secrete BM components such as laminins, type IV collagen, and perlecan. However, generally most of this material is released in the medium in 2D cultures (except BHK forming a BM- like surface coat). This can be dramatically improved in 3D-cocultures with stromal cells like fibroblasts which provide other ECM or BM molecules like nidogens as adapter or crosslinking molecules. You find more details and references for example in our review paper (Breitkreutz et al., Biomed Res Int 2013: 179784) I have attached for your convenience. Fundamental work on normal breast epithelial cells and the role of ECM has been done by Mina Bissell which includes changes with tumor progression as well. In this context also the activities of ECM degrading proteases are of crucial importance like MMP-2, -9 and -14. Another aspect is that conventional serum containg media provide the ECM proteins fibronectin (also made by fibroblasts) and vitronectin being usually crucial for cell attachment.
All my best for 2016 to everybody as well, Dirk Breitkreutz
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Yes they secrete ECM that is different across cell lines. To study the ECM released, you can plate cells for a day or two and then detach them with EDTA (not with Trypsin) - Versene from ThermoFisher is a cell culture EDTA solution we used: That will leave the cell-free ECM on the dish and you can then harvest that e.g. with SDS or Laemmli buffer for further analyses. Thus, you can evaluate the differences in the ECM.
Yes, they produce well their extracellular matrix in their tumor microenvironment, with specific biochemical and biomechanical properties.
For more Infos, see please (Review):
Pengfei Lu, Valerie M. Weaver, and Zena Werb (2012).The extracellular matrix: A dynamic niche in cancer progression. JCB vol. 196 no. 4 395-406
Regards
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