Anton Wellstein

5 Questions 10 Answers 0 Followers

Questions related from Anton Wellstein

Does anybody have a protocol to isolate, grow and expand cancer cells from leukapheresis of cancer patients ?

Does anybody have a protocol and successfully isolated, grown and expanded cancer cells from leukapheresis of patients with solid tumors such as breast, colon, pancreatic, lung cancer ? Lombardi...

01 January 2015 10,075 1 View

How can we test for compatibility of allograft tumor cell lines from a mixed mouse strain in mixed mouse strains ?

We generated clonal cell lines from p48-Cre X mutKras/mutp53 mouse lines and use these lines as allografts. Due to the strain mix in the donor line, we expected and do see some rejection of the...

09 September 2014 8,792 3 View

Can anyone help with deep sequencing of a PCR product?

We generated PCR products of different lenghts ( 200 and 2,000 nts ) from different types of assays: RACE-PCR, cDNA library inserts, amplicons from mutated DNA sites in tumors with heterogeneous...

08 August 2014 6,706 9 View

Any suggestions on antibodies or stains to detect and identify immune cell infiltrates in mouse tissue sections (normal or tumor)?

Technique to detect immune infiltrates in mouse tissue sections. Preferably formalin-fixed, paraffin-embedded (FFPE).

06 June 2014 3,442 0 View

How can I best recover viable cells from a brain tumor specimen that was frozen at the time of surgery?

We wish to recover cells from a brain tumor specimen that were frozen at the time of surgery and wonder if someone has a suggestion on a protocol.

04 April 2014 1,840 1 View