Pooja Patheja

6 Questions 3 Answers 0 Followers

Questions related from Pooja Patheja

How do people from so many institutes collaborate for research?

Usually there are 20 or more authors from five or more universities in a single research article published in Nature or Cell. How does this collaboration works? How to get into such collaborations?

01 January 2016 4,983 6 View

Do cancer cells secrete extra cellular matrix in monolayer?

I want to know whether cancer cells like MCF-7, MBA-MB 231, Colo etc. secrete ECM when cultured in monolayer? If yes, what is molecular composition of such matrix? Any reference literature...

12 December 2015 3,221 5 View

What transwell inserts are suitable to study cell migration and invasion?

I need to perform cell migration assay using transwell inserts of 8 micron pore size membrane and matrigel. What is most preffered membrane for such experimets: Polycarbonate, PTEF or PET?

04 April 2015 8,503 5 View

What are methods to concentrate cell supernatant for gelatin zymography?

I have to perform gelatin zymography from cell supernatant. I pellet down the cells and collect supernatant. After that how should I proceed? How do I concentrate the supernatant?

12 December 2014 8,246 4 View

What will happen to cells if I do not add insulin to the culture medium?

I have received Mcf-7 and MBA-MD 231 cells from cell repository. The media composition provided in pamphlet received says 0.01mg/ml insulin added to the medium. I don't have insulin at present...

08 August 2013 5,851 5 View

What is the p53 status of MCF-7, MDA-MB-231, MDA-MB-435 cell lines?

I have to perform experiments with p53 wild type and mutant breast cancer cell lines for which I need to know their p53 status. Can anyone tell me the p53 status (WT or Mutant) of MCF-7,...

05 May 2013 8,960 8 View