What's the best way to detect the extracted lipids in order to confirm that your lipid extraction protocol is correct?

I am extracting Lipids from RBC Membranes using the Cholorform/Methanol (2:1) method. After phase separation and consequent evaporation of the organic phase, what would be the best way to confirm...

11 December 2019 9,404 2 View

How to isolate cell membrane from K562 cell lines?

I want to extract the the degraded plasma membrane of K562 cell lines without any of the cell organelles or extracellular constituents as I want to use the membrane for lipidomic analysis.

03 April 2019 408 3 View

From where can I get antibody against Merozites Surface Antigen 1 of Babesia bovis?

Babesia bovis is a parasite of cattles which is present in RBCs of the host. Can anyone tell me from where can I order commercially available antibody for Merozites Surface Antigen-1 of Babesia...

05 June 2018 4,107 0 View

Do we need to enrich miRNA from total RNA?

I want to know whether miRNA studies can be carried out on total RNA extracted from samples or do you absolutely need to enrich small RNAs?

04 May 2018 9,001 2 View

Does anyone have any idea regarding Qiagen RNeasy Protect Saliva Mini Kit?

I want to check expression levels of miRNAs in saliva samples. I came across this kit and it provides protecting agent for the transport of Samples and a kit for total RNA extraction. My Question...

04 May 2018 1,431 2 View

RNA extraction from Plant Tissues?

Any replacement for DTT and PVP in RNA Extraction protocol from kit of Thermofischer?

03 April 2018 8,389 4 View

DNA Quantification on Thermoscientific Multiskan Go?

I quantified the extracted DNA on Multiskan Go. The provider of the instrument directed me to take the absorbance at 260 and multiply it by 1000. According to him this would give the concentration...

02 March 2018 6,929 2 View

Lypholizing DNA Extraction samples?

I have extracted DNA from animal tissue using a kit from vivantis, but the quantification revealed very low concentration. Now I want to lypholize all the samples so as to concentrate total DNA...

02 March 2018 227 6 View

Saliva Sampling for RNA Studies?

I want to do miRNA profiling in Oral Squamous Cell Carcinoma. Sampling for this will be done in another city and to bring it to lab it will take one day for the courier. Now can I use RNA Later to...

02 March 2018 1,663 2 View

Can I perform histopathology on cell lines?

I have transfected cell line with an inhibitor of a particular gene. that gene promotes tumor growth. Now I want to check whether the inhibitor has caused change in the tumor growth. So can I do...

01 February 2018 7,420 7 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

No title

02 March 2021 3,060 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View