I quantified the extracted DNA on Multiskan Go. The provider of the instrument directed me to take the absorbance at 260 and multiply it by 1000. According to him this would give the concentration of DNA in ug/ml. I read somewhere else that it is actually the difference in A260 and A320 which is then multiplied by dilution factor and in turn multiplied by 50 which will give concentration. Could someone tell me what is the correct way and if later is correct then what dilution factor is he referring to?
Hi, I have the same question to quantify DNA conc. using multiscan Go, have you figured it out now? Thank you!
Yes. For quantification you have to take the reading at 260nm wavelength and then select the basic calculation option. There check the multiplication option and then select 260nm from the dropdown menu. Enter 1000 as the multiplication number. It will give you the Concentration in microgram/ml or ng/microliter. If there still is problem you could read the file I am attaching which will give you the step by step instructions.
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