12 Questions 20 Answers 0 Followers
Questions related from Muhammad Behroz Naeem Khan
I am extracting Lipids from RBC Membranes using the Cholorform/Methanol (2:1) method. After phase separation and consequent evaporation of the organic phase, what would be the best way to confirm...
12 December 2019 9,478 2 View
I want to isolate Plasma membrane only from the K562 cell lines to for lipidomics. Can anyone provide me with a protocol that i can use that shows how to first prepare the gradient and then in...
05 May 2019 9,921 1 View
I want to extract the the degraded plasma membrane of K562 cell lines without any of the cell organelles or extracellular constituents as I want to use the membrane for lipidomic analysis.
04 April 2019 473 3 View
Babesia bovis is a parasite of cattles which is present in RBCs of the host. Can anyone tell me from where can I order commercially available antibody for Merozites Surface Antigen-1 of Babesia...
06 June 2018 4,166 0 View
I want to know whether miRNA studies can be carried out on total RNA extracted from samples or do you absolutely need to enrich small RNAs?
05 May 2018 9,058 2 View
I want to check expression levels of miRNAs in saliva samples. I came across this kit and it provides protecting agent for the transport of Samples and a kit for total RNA extraction. My Question...
05 May 2018 1,488 2 View
Any replacement for DTT and PVP in RNA Extraction protocol from kit of Thermofischer?
04 April 2018 8,449 4 View
I want to do miRNA profiling in Oral Squamous Cell Carcinoma. Sampling for this will be done in another city and to bring it to lab it will take one day for the courier. Now can I use RNA Later to...
03 March 2018 1,735 2 View
I have extracted DNA from animal tissue using a kit from vivantis, but the quantification revealed very low concentration. Now I want to lypholize all the samples so as to concentrate total DNA...
03 March 2018 291 6 View
I quantified the extracted DNA on Multiskan Go. The provider of the instrument directed me to take the absorbance at 260 and multiply it by 1000. According to him this would give the concentration...
03 March 2018 7,031 2 View
I have transfected cell line with an inhibitor of a particular gene. that gene promotes tumor growth. Now I want to check whether the inhibitor has caused change in the tumor growth. So can I do...
02 February 2018 7,493 7 View
I have isolated total RNA from blood samples of HBV by trizol method, converted it into cDNA and will now perform Real Time PCR for expression analysis of selected miRNAs by using the specific...
05 May 2017 1,705 5 View
