Ying Wang

3 Questions 11 Answers 0 Followers

Questions related from Ying Wang

Could someone shares their protocol and experience in isolating mtDNA-enriched DNA from mouse tails?

Hello, I am looking for a good protocol for isolating mtDNA-enriched DNA from mouse tails for genotyping. I tried the Qiagen tissue and blood kit but it gave me unreliable mtDNA yield. I know...

01 January 2019 3,076 5 View

Can anyone please share a protocol for genotyping the mutator mouse (PolgAD257A)?

Hello, I have troubles with genotyping the mutator mouse (PolgAD257A). I use a total DNA extraction kit to extract DNA from mouse tails and then use a hotstart Taq in the genotyping PCR. My other...

09 September 2018 4,894 2 View

Should I use 0.1M PBS or 0.01M PBS (1xPBS) for IHC washing and incubation steps?

I am new to IHC. I looked over several protocols for IHC of tyrosine hydroxylase (TH) and saw that some use 1XPBS, some use 0.1M PBS (10X), or PBS+Triton X-100, or TBS, for rinsing sections and...

12 December 2017 395 2 View