Babesia bovis is a parasite of cattles which is present in RBCs of the host. Can anyone tell me from where can I order commercially available antibody for Merozites Surface Antigen-1 of Babesia bovis? I need it for my research.
I am extracting Lipids from RBC Membranes using the Cholorform/Methanol (2:1) method. After phase separation and consequent evaporation of the organic phase, what would be the best way to confirm...
11 December 2019 9,404 2 View
I want to isolate Plasma membrane only from the K562 cell lines to for lipidomics. Can anyone provide me with a protocol that i can use that shows how to first prepare the gradient and then in...
04 May 2019 9,861 1 View
I want to extract the the degraded plasma membrane of K562 cell lines without any of the cell organelles or extracellular constituents as I want to use the membrane for lipidomic analysis.
03 April 2019 408 3 View
I want to know whether miRNA studies can be carried out on total RNA extracted from samples or do you absolutely need to enrich small RNAs?
04 May 2018 9,001 2 View
I want to check expression levels of miRNAs in saliva samples. I came across this kit and it provides protecting agent for the transport of Samples and a kit for total RNA extraction. My Question...
04 May 2018 1,431 2 View
Any replacement for DTT and PVP in RNA Extraction protocol from kit of Thermofischer?
03 April 2018 8,389 4 View
I quantified the extracted DNA on Multiskan Go. The provider of the instrument directed me to take the absorbance at 260 and multiply it by 1000. According to him this would give the concentration...
02 March 2018 6,929 2 View
I have extracted DNA from animal tissue using a kit from vivantis, but the quantification revealed very low concentration. Now I want to lypholize all the samples so as to concentrate total DNA...
02 March 2018 227 6 View
I want to do miRNA profiling in Oral Squamous Cell Carcinoma. Sampling for this will be done in another city and to bring it to lab it will take one day for the courier. Now can I use RNA Later to...
02 March 2018 1,663 2 View
I have transfected cell line with an inhibitor of a particular gene. that gene promotes tumor growth. Now I want to check whether the inhibitor has caused change in the tumor growth. So can I do...
01 February 2018 7,420 7 View
Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...
03 March 2021 5,999 3 View
Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...
02 March 2021 10,003 2 View
Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...
01 March 2021 3,867 3 View
We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...
01 March 2021 4,937 1 View
I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....
01 March 2021 3,607 3 View
Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?
28 February 2021 1,577 1 View
I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.
28 February 2021 6,512 3 View
When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...
28 February 2021 2,133 3 View
I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.
28 February 2021 5,929 2 View
I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...
25 February 2021 8,404 3 View