Babesia bovis is a parasite of cattles which is present in RBCs of the host. Can anyone tell me from where can I order commercially available antibody for Merozites Surface Antigen-1 of Babesia bovis? I need it for my research.
I am extracting Lipids from RBC Membranes using the Cholorform/Methanol (2:1) method. After phase separation and consequent evaporation of the organic phase, what would be the best way to confirm...
11 December 2019 9,507 2 View
I want to isolate Plasma membrane only from the K562 cell lines to for lipidomics. Can anyone provide me with a protocol that i can use that shows how to first prepare the gradient and then in...
04 May 2019 9,956 1 View
I want to extract the the degraded plasma membrane of K562 cell lines without any of the cell organelles or extracellular constituents as I want to use the membrane for lipidomic analysis.
03 April 2019 506 3 View
I want to know whether miRNA studies can be carried out on total RNA extracted from samples or do you absolutely need to enrich small RNAs?
04 May 2018 9,086 2 View
I want to check expression levels of miRNAs in saliva samples. I came across this kit and it provides protecting agent for the transport of Samples and a kit for total RNA extraction. My Question...
04 May 2018 1,510 2 View
Any replacement for DTT and PVP in RNA Extraction protocol from kit of Thermofischer?
03 April 2018 8,481 4 View
I quantified the extracted DNA on Multiskan Go. The provider of the instrument directed me to take the absorbance at 260 and multiply it by 1000. According to him this would give the concentration...
02 March 2018 7,066 2 View
I want to do miRNA profiling in Oral Squamous Cell Carcinoma. Sampling for this will be done in another city and to bring it to lab it will take one day for the courier. Now can I use RNA Later to...
02 March 2018 1,765 2 View
I have extracted DNA from animal tissue using a kit from vivantis, but the quantification revealed very low concentration. Now I want to lypholize all the samples so as to concentrate total DNA...
02 March 2018 319 6 View
I have transfected cell line with an inhibitor of a particular gene. that gene promotes tumor growth. Now I want to check whether the inhibitor has caused change in the tumor growth. So can I do...
01 February 2018 7,525 7 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...
31 July 2024 4,510 3 View
I went for Plackett Burman Design for selecting the optimization factors of growth conditions for pesticide degradation. I started with 7 parameters. But, after the experiments and analysis, only...
29 July 2024 5,015 1 View
In hirshfeld surface calculation i did not find any red spot in hirshfeld surface, is it ok for research article to write?
28 July 2024 2,988 0 View
Dear researchers, Why specific capacitance is not proportional to the specific surface area? I have material A with a surface area higher than material B, but material B is showing little bit...
26 July 2024 9,156 2 View
I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...
23 July 2024 6,664 6 View
I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...
22 July 2024 9,160 2 View
Hello, I recently stained my cells with an Arf6 antibody. The healthy control looks pretty normal, while the disease group cells have abnormally large black structures in the cell body. Has anyone...
21 July 2024 1,044 1 View
Hi everyone, I have a question regarding surface and interfacial tension. Although I understand that these tensions are studied for different purposes, can they be considered equivalent in the...
18 July 2024 382 5 View