I want to do miRNA profiling in Oral Squamous Cell Carcinoma. Sampling for this will be done in another city and to bring it to lab it will take one day for the courier. Now can I use RNA Later to prevent RNA from degradation in Saliva? If yes then how will I get the supernatant of Saliva if RNA Later is already mixed with it? Is it possible to centrifuge it with RNA Later Present? Or is there any other method to carry out the sampling in this case?
Dear Sir. Concerning your issue about Saliva Sampling for RNA Studies . I think the following below links and the attached file may help you in your analysis:
https://www.karger.com/Article/Pdf/328026
https://www.ncbi.nlm.nih.gov/pubmed/23564756
Thanks
Hi Muhammad,
I had worked in a human saliva biobanking, oral cancer biomarker project. We had used multisite sample collection. So we had to manage the RNA stabilization during sample-to-process timeframe. We have esthablised a system for saliva RNA stabilization. We use PreAnalytiX PAXgene Tube (https://www.preanalytix.com/products/blood/RNA/paxgene-blood-rna-tube?suggest=1). It is use for human venous blood sample originally. (In another project we have used them to stabilize RNA in blood with success.) You just pull out the cap of the tube. It contains 7.5 ml stabilization reagent. We use 1 ml room temp. PAXgene reagent per 0.2 ml saliva (during collection it has stored on ice). After adding of the saliva sample just mix it throughly and store on ice, untill RNA isolation or you can froze at -20 °C for 24 h followed optional storage at -80 °C for long term storage.
Bests,
Tamas
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