29 October 2020 3 7K Report

Please suggest portals to retrieve normalized-quantile expressions of micro-RNAs (in RPM).My aim is to construct a correlation plot between the gene of interest and microRNA. I searched in human miRNA tissue atlas and DASHR. But Iam unable to find the values for many miRNAs

More Anupama Vijayakumar's questions See All
RDF calculation of water inside the Carbon nanotubes?

I am trying to calculate the rdf of water molecules present inside the Carbon nanotube. I tried to do the indexing first using gromacs tool gmx selection. But when it comes to calculating rdf, I...

17 February 2021 1,066 3 View

How to change the protein chain names X into A, B, C, D after MD simulations in Gromacs?

I am running MD simulations for holoenzyme in Gromacs. The protein has four chains like A, B, C, D. After MD simulations, All the chains are showing as X chain in VMD and even in the PDB file....

12 November 2020 6,228 1 View

How to filter few markers from a gene set?

I am not good in bioinformatics. I have a gene set which has some 1000 genes which are highly correlating with aging. I want to select some highly significant genes from this geneset for further...

04 November 2020 5,602 5 View

Hello Can anyone suggest a good way of inducing oxidative stress in mice to exhibit proteinurea phenotype in the mice?

Hello Can anyone suggest a good way of inducing oxidative stress in mice to exhibit proteinurea phenotype in the mice or a good method to induce oxidative stress in the mice to study kidney...

26 July 2020 2,999 3 View

I am trying to do an article on domestic migration. Can anyone suggest me a research gap area where I can do my research on?

We have seen statistically that the domestic migration of workers are increasing every year. People are moving from rural to urban areas for better work and life. This migration has brought in...

03 March 2020 5,445 4 View

How will artificial intelligence help to develop MSMEs ?

Artificial Intelligence is progressive and has wider impact on various sectors. I would like to know how AI is impacting Micro, Small and Medium enterprises. The ways in which it is enacting for...

30 January 2020 7,367 3 View

What will be the perfect markers to differentiate Neural Stem cells from differentiated Neurons?

I've been working on differentiating Rat fetal Neural Stem cells into Neurons with a drug of interest. The Problem here for me is I have used various Neuronal markers like MAP2, TUJ1, NFM to...

15 October 2019 7,114 5 View

Autophagy: After how many days of fasting does autophagy usually start?

Hi, Could you please help me to explore after how many days of fasting does autophagy actually start in (1) Humans (2) Animal models and/or (3) cell lines, please? Thank you in advance

30 June 2019 8,778 5 View

Is there any standarad method or procedure for estimation of water productivity or water use efficiency of integrated farming system?

In integrated farming system (IFS), water applied for one component is also utilized in another component. So how we can compute the WUE and Water productivity of any IFS?

01 April 2019 7,555 2 View

Why the SOD1 mutant model expressing the mutant protein but not the native protein?

I am working with drug activity on familial ALS model cells. I am using mouse Neural stem cells which express the mutated human SOD1 protein. I did western blot with the protein extracted from the...

14 March 2019 511 3 View

Similar questions and discussions
MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Suggest me some trending topics for phd research in text mining/AI/machine learning?

My master's research is in info retrieval and text mining. I would be grateful if you could help me to select a good topic for my phd research proposal.

24 February 2021 393 14 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View

Are exogenous molecules transferred between human cells during doubling ?

Hello RG community, I wonderer whether exogenous molecules (e.g. reporter plasmids, miRNAs or other artificial molecules) are transferred between cultured human cells during their doubling ? So,...

21 February 2021 6,633 3 View

Does qualitative research using closed and open ended survey and semi structured interviews qualify as triangulation?

I am using qualitative research to look at employee motivation. I will use a closed and open ended survey (for biodata information and general views to motivation) and semi structured interviews...

18 February 2021 436 11 View

Has anyone encountered any problems with abm good microRNA primers for quantification of mature miRNAs?

I've been trying to quantitate mature microRNAs in 293T cells with and without 'X'. 2ug of total RNA (isolated by Trizol method) is converted to cDNA using All-in one microRNA cDNA synthesis kit...

17 February 2021 8,986 2 View

How to find standard axial compressor blade data files ?

I'm looking for standard axial compressor blade row data files for CFX simulation , so could somebody tell me where can I find them ? Like rotor37 , PBS rotor , and something else about them...

13 February 2021 7,745 3 View

How we can get maximum benefit from Big Data utilization in our life?

Big data is a field that treats ways to analyze, systematically extract information from, or otherwise deal with data sets that are too large or complex to be dealt with by traditional...

13 February 2021 7,347 6 View

How to model in HFSS or FEKO Free Space Material Characterization?

I am having trouble setting up this problem in HFSS or FEKO. In HFSS, I've tried both FEM and FEBI-IE solvers. With FEKO, I've tried it's MoM solver and default settings. I am would like model...

09 February 2021 375 2 View

What is the best way to fix plasma derived miRNA for subsequent cDNA synthesis?

I need to quantify blood plasma derived miRNA through q-RT-PCR. For this I need to fix RNA content in my cDNA reaction. What is the best way to do this? Currently, I take readings of my plasma...

03 February 2021 9,330 3 View

Our partners will collect data and use cookies for ad personalization and measurement. Learn how we and our ad partner Google, collect and use data. Agree & close