Hello!

I planned to do an multiplex RNAscope fluorescent assay for cultured cells in a 96 well plate format according to the protocol provided by ACD: https://acdbio.com/sites/default/files/SOP%2045-009A_Tech%20Note%20Mux%20FL_CulturedCells_96%20well-plateV2_10112017.pdf)

This protocol does not include an antigen retrieval, which is normally needed to enable the binding of the probes to the target RNA. Does anyone tried this protocol?

Thanks for your help!

Thea

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