i mean are chemical compounds of lysis buffer able to damage, probably.
lysis solution include: (2.5 M NaCl, 0.1 M EDTA, 10 mM Tris, 1% Triton X-100, 9% DMSO, pH 10)
no I havent control, i carried out comet assay on fish gill and liver tissue and used lysis buffer for all samples( treatment and control).
now, i want know that lysis buffer can making DNA damage and creat error in result?
No, lysis buffer will not cause DNA damage. Lysis buffer disrupts all proteins, RNA and membranes which then diffuse into the agarose matrix. DNA of the cell remains for analysis.
I agree with Kamran Shekh, however extended lysis of the cells may affect quality of agarose gels.
Hi.
I also don't think the lysis buffer in itself can cause DNA damage.
However, it's always good to have controls (without treatment) to compare your results.
If the control has 0 to highest 10% DNA damage (background damage), then your buffer and procedure is fine.
The control(s) is the best judge of your procedure and solutions.
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