How can I quantify liver tissue disorders in fish ?

04 May 2015 4,475 4 View

Can the lysis buffer (high salt) cause DNA damage in cells?

04 May 2015 5,364 7 View

Why small and immature fish used are preferably used in aquatic toxicology studies?

i.e. Instead of using the big and mature fish, small and immature fish such as juvenile and fingerling used

01 February 2015 1,348 11 View

What are the best conditions for capture imaging by microscope in the micronucleus assay?

Micronucleus assay to be carried out on fish. I am attaching several images, Please tell us your opinion.

31 December 2014 5,360 5 View

Are these a good images for comet assay test?

Recently I have captured new images with fluorescent microscope. I attached several images, can you comment on these?

31 December 2014 1,823 11 View

Does anyone what is the best cutting Thickness for microtome?

i want cutting the gill and liver tissue of fish

31 December 2014 5,637 13 View

What concentration of ethidium bromide dye should be used in slides staining for the comet assay method?

31 December 2014 5,155 3 View

Can anyone suggest a protocol for comet assay on some tissues of fish?

in fact I want using gill and liver cells.

31 December 2014 2,259 2 View

What is the best method for determination the sex of immature goldfish?

these fish are not mature and me want to separation it for propagation

31 December 2014 8,468 3 View

Can anyone help me with a paper about exposing toxic substances on fish red blood cells in FBS Medium?

i want expose toxicant substances on fish red blood cells.

11 December 2014 3,223 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View