Can the lysis buffer (high salt) cause DNA damage in cells?

04 May 2015 5,364 7 View

Why small and immature fish used are preferably used in aquatic toxicology studies?

i.e. Instead of using the big and mature fish, small and immature fish such as juvenile and fingerling used

01 February 2015 1,348 11 View

What are the best conditions for capture imaging by microscope in the micronucleus assay?

Micronucleus assay to be carried out on fish. I am attaching several images, Please tell us your opinion.

31 December 2014 5,360 5 View

Are these a good images for comet assay test?

Recently I have captured new images with fluorescent microscope. I attached several images, can you comment on these?

31 December 2014 1,823 11 View

Does anyone what is the best cutting Thickness for microtome?

i want cutting the gill and liver tissue of fish

31 December 2014 5,637 13 View

What concentration of ethidium bromide dye should be used in slides staining for the comet assay method?

31 December 2014 5,155 3 View

Can anyone suggest a protocol for comet assay on some tissues of fish?

in fact I want using gill and liver cells.

31 December 2014 2,259 2 View

What is the best method for determination the sex of immature goldfish?

these fish are not mature and me want to separation it for propagation

31 December 2014 8,468 3 View

What is the best method for doing comet assay test on fish sperm?

if possible, attaching a file for this method please.

31 December 2014 1,782 3 View

Can anyone help me with a paper about exposing toxic substances on fish red blood cells in FBS Medium?

i want expose toxicant substances on fish red blood cells.

11 December 2014 3,223 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View

Experimental Design: How deal with pseudreplicates with immunological and clinical-chemical paramaeters?

Hello everybody, I am investigating the influence of a toxinon various clinical-chemical and haematological parameters in fish. I have three treatments (control, low dose, high dose) in my...

24 February 2021 1,243 1 View

Hi i want to ask that in order to find out LC 50 (96 hours) of particular toxin for fish. Should we keep the animals with food or without food?

if we expose the fish after acclimatization to particular toxin in order to find out LC 50 (96 hours).Should we keep the fish off feed or with feed for that duration. Some literature show that...

24 February 2021 357 2 View

Measuring Total immunoglobulin whole-body of fish larvae?

Hi Dear All, I prepared the extract of fish larvae whole body with PBS. Then protein concentration of the whole-body extraction samples determined by the Folin-Ceiocalteu-Phenol method (Lowry...

17 February 2021 1,971 3 View

What is the identity of additional bands found during fish gut microbiome 16S V4 PCR amplification?

Hi, I am carrying out test PCRs for 16S amplicon sequencing on Illumina MiSeq. I am using standard V4 primers: 515F/806R. The aim is to profile the microbiome of fish guts under different...

16 February 2021 5,668 6 View

Is this the fragment of a sauropod tooth?

Dear colleagues. Fragments of this species are very rare from a terrestrial Lower Cretaceous locality from Germany. Up to now from this locality sauropods could not be proved with certainty. Other...

14 February 2021 2,310 1 View

Despite single-cell RNA, how can I characterize a new cell subtype/phenotype?

A new cell subtype was found out only during regeneration phases after a cuprizone-induced demyelination model. How can I characterize this cell? It is important to highlight that was already...

13 February 2021 1,245 1 View

Does diseases like fungal,bacterial etc of rice plant is different from nutrient deficiency diseases?how deep learning is used except classification?

can any one suggest me other than classification, how deep learning technique can be used in plant disease detection in rice plant? could a research work continued from fungal,bacterial...

07 February 2021 470 2 View

Problem to dissolve 50%wt Glycerol in 4%wt PVOH 28-99 ??

Hello everyone, I am trying to prepare a solution out of PVA(Polyvinyl alcohol)_Glycerol_Wasser . (4% PVA 28-99 and 50% Glycerin) To prepare this solution I first add PVA to 80degree water and...

06 February 2021 2,300 3 View