Can anyone suggest a protocol for comet assay on some tissues of fish?

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Patrick Heinrich

Hey,

if you are looking for a protocol, you may start here:

http://www.ncbi.nlm.nih.gov/pubmed/11386733

Here, the cells are taken into culture before exposure, but you might skip that and expose the fish directly.

As a start for preparation of cell suspensions from tissues, maybe take a look at that one, too:

http://www.ncbi.nlm.nih.gov/pubmed/16916029

Hope I could help you getting started.

Santoshk Prajapati

Please find the protocol