How can I quantify liver tissue disorders in fish ?

04 May 2015 4,475 4 View

Can the lysis buffer (high salt) cause DNA damage in cells?

04 May 2015 5,364 7 View

Why small and immature fish used are preferably used in aquatic toxicology studies?

i.e. Instead of using the big and mature fish, small and immature fish such as juvenile and fingerling used

01 February 2015 1,348 11 View

What are the best conditions for capture imaging by microscope in the micronucleus assay?

Micronucleus assay to be carried out on fish. I am attaching several images, Please tell us your opinion.

31 December 2014 5,360 5 View

Are these a good images for comet assay test?

Recently I have captured new images with fluorescent microscope. I attached several images, can you comment on these?

31 December 2014 1,823 11 View

Does anyone what is the best cutting Thickness for microtome?

i want cutting the gill and liver tissue of fish

31 December 2014 5,637 13 View

What concentration of ethidium bromide dye should be used in slides staining for the comet assay method?

31 December 2014 5,155 3 View

What is the best method for determination the sex of immature goldfish?

these fish are not mature and me want to separation it for propagation

31 December 2014 8,468 3 View

What is the best method for doing comet assay test on fish sperm?

if possible, attaching a file for this method please.

31 December 2014 1,782 3 View

Can anyone help me with a paper about exposing toxic substances on fish red blood cells in FBS Medium?

i want expose toxicant substances on fish red blood cells.

11 December 2014 3,223 2 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

How does the gut microbiome affect the fibrotic process within the liver?

and Can the gut microbe be used for the treatment or diagnosis of liver fibrosis?

02 March 2021 557 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View