Milad Esmaeilbeigi

15 Questions 21 Answers 0 Followers

Questions related from Milad Esmaeilbeigi

How can I quantify liver tissue disorders in fish ?

for example in gill tissue, considering number of lamella(each treatment 500 lamella).

05 May 2015 4,551 4 View

Can the lysis buffer (high salt) cause DNA damage in cells?

i mean are chemical compounds of lysis buffer able to damage, probably. lysis solution include: (2.5 M NaCl, 0.1 M EDTA, 10 mM Tris, 1% Triton X-100, 9% DMSO, pH 10)

05 May 2015 5,455 7 View

Why small and immature fish used are preferably used in aquatic toxicology studies?

i.e. Instead of using the big and mature fish, small and immature fish such as juvenile and fingerling used

02 February 2015 1,588 11 View

Are these a good images for comet assay test?

Recently I have captured new images with fluorescent microscope. I attached several images, can you comment on these?

01 January 2015 1,906 11 View

Can anyone suggest a protocol for comet assay on some tissues of fish?

in fact I want using gill and liver cells.

01 January 2015 2,331 2 View

What is the best method for determination the sex of immature goldfish?

these fish are not mature and me want to separation it for propagation

01 January 2015 8,567 3 View

What is the best method for doing comet assay test on fish sperm?

if possible, attaching a file for this method please.

01 January 2015 1,865 3 View

What concentration of ethidium bromide dye should be used in slides staining for the comet assay method?

i used 10 microliter ethidium bromide for each loaded samples(two) on slide(i.e 20 microliter for each slide) but under the fleurosent microscope, can hardly see the halo DNA or comet. One of the...

01 January 2015 5,229 3 View

Does anyone what is the best cutting Thickness for microtome?

i want cutting the gill and liver tissue of fish

01 January 2015 5,716 13 View

What are the best conditions for capture imaging by microscope in the micronucleus assay?

Micronucleus assay to be carried out on fish. I am attaching several images, Please tell us your opinion.

01 January 2015 5,473 5 View

After the ethidium bromide staining in comet assay slides, how much time do we have to capture images by fluorescent microscope?

i mean: Destruction slides by light decomposition

12 December 2014 6,509 4 View

Can anyone help me with a paper about exposing toxic substances on fish red blood cells in FBS Medium?

i want expose toxicant substances on fish red blood cells.

12 December 2014 3,295 2 View

Can anyone tell me the mechanism for micronucleus formation in fish blood cells?

I would like to know exactly what happens in the cell cycle?

12 December 2014 6,762 16 View

Is there possible to create a slide archive of etidium bromide staining (such as silver staining) for comet assay?

as regards that etidum bromide stain is very sensitive to light

12 December 2014 2,946 4 View

In the silver staining method I do not see anything under the microscope, does anyone know why?

in slides under microscope, only parts of the tissue or other things are found and the comet tail is not found.

12 December 2014 4,186 3 View