Kamran Shekh

10 Questions 28 Answers 0 Followers

Questions related from Kamran Shekh

What is the typical workflow of mutant protein expression in mammalian cells?

I want to express some variants of human anion-exchanger transporter protein in K562 cell line using viral transfection method. What is the typical workflow for achieving this. No need to go into...

11 November 2019 5,809 3 View

Can we store isolated organelles in -80?

I will isolate organelles such as mitochondria and lysosomes from fish gill with the intention of measuring marker enzymes within these organelles. Can we store these isolated organelles in -80°...

08 August 2019 4,477 3 View

Is it possible to extract protein from animal tissue by homogenizing in water only, without any lysis buffer or salt?

The objective is to simply quantify the protein by Bradford method. Is it possible to just homogenize the tissue in water, centrifuge it and analyse the supernatant with Bradford's reagent?

06 June 2018 2,349 5 View

How to convert a test tube assay to microplate assay?

I am trying to measure Ascorbic acid levels in tissue using Omaye et al protocol (Methods in Enzymology, Volume 62, 1979, Pages 3-11). This method is based on reduction of ferric chloride and...

01 January 2018 9,319 6 View

How to do statistical analysis of the enzyme kinetics parameters (Km and Vmax) among several treatment groups?

Lets say I measure kinetics of an enzyme under three toxic chemical exposure conditions. After fitting the enzyme velocity data using Michaelis Menten model, I am able to get the kinetic...

12 December 2016 4,200 9 View

GeNorm in NormqPCR package of R?

In the original geNorm VBA applet, the raw Ct values were required to be converted in the relative Ct values (by using PCR efficiency and lowest Ct among samples). Does 'selectHKs' function in...

09 September 2016 8,061 5 View

Are 'predicted' protein sequences useful for multiple alignment in order to draw conclusions about conserved domains?

I was wondering if predicted protein sequences, which sometimes come out of the 'Gnomon annotation program' of NCBI, can be used for multiple sequence alignment in common tools such as Clustal...

03 March 2016 2,462 1 View

What will be the impact of increased enzyme expression on its Vmax and Km?

Will increased enzyme expression lead to increase in Vmax ? Ideally, it should be. What is your opinion on this?

08 August 2015 6,077 12 View

How do I remove skin from small fishes?

Is there any specific protocol for removing the skin from small fishes (

07 July 2015 2,203 8 View

Is it possible to statistically compare two values if we do not have information on replicates?

E.g. Is it possible if we want to check statistical significance in the difference between two separately published LC50 for a particular chemical in two separate species? To elaborate lets take...

06 June 2015 4,938 18 View