I have been using BSA to do control experiments on a plasmonic biosensor with gold nanoparticles of various coatings. BSA tends to stick irreversibly to our nanoparticles because of its hydrophobic regions and surface thiol, and probably due to electrostatics as well.
We'd like to repeat these controls with a less "sticky" protein; that is, a protein that's unlikely to have surface thiols and ideally not very many hydrophobic regions. We'd like this protein to be of similar molecular weight (ie 40-85kDA) and be affordable. Would anyone have any suggestions? This would help me tremendously.
No, I haven't. We ended up using neutravidin. Can you elaborate on your thinking about a cellulose derivative?
any protein which contains hydrophobic amino acids will stick to the hydrophobic surfaces, So it's will be difficult to find a protein without this AA.
Therefore try HES (Hydroxyethyl starch). It was used as plasma expander in intensive care. Use it also in concentrations like BSA.
Sodium carboxymethyl cellulose has an average MW of 90K. It is relatively inexpensive and sold in various forms from chemical producers worldwide. Dow is a major producer that will customize to meet your needs.
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