Jeff Nelson

5 Questions 11 Answers 0 Followers

Questions related from Jeff Nelson

Magnesium stearate solubility in DMSO?

Trying to determine if MgSt is soluble in DMSO. I am using DMSO to solubilize two APIs that are insoluble in water. Once the APIs are solubilized, I will dilute the DMSO-API solution in water to...

05 May 2018 353 3 View

What is the best way to quantify the specific antibodies against the venom in my neutralization assay?

I need help with a neutralization assay of chicken IgY incubated with rattlesnake venom. I have hyperimmune chickens that have been vaccinated with venom. I have the purified IgY. I have tried...

08 August 2014 3,410 8 View

Is IgY non-specific and if so how do I overcome the non-specific binding?

I am trying to develop an ELISA that is coated with an antigen and specifically binds IgY. What I am seeing is that a control IgY is non-specifically binding as well. Is IgY non-specific? If so...

04 April 2014 1,645 3 View

Extraction of glycerol from restriction enzymes

What is the best method for extraction of glycerol? Dialysis seems to be lowering the activity of the enzymes.

08 August 2012 6,700 2 View

I have immunoglobulins from canine plasma, but they are contaminated with albumin. What is the best single step to remove albumin?

I do not have access to chromatography. I have read that zinc or copper will precipitate the albumin.

10 October 2011 8,112 6 View