How can I make sure that BCTC doesn't fall out of solution/form DMSO crystals when treating cells?

I resuspended my BCTC as described on the bottle at 10mg/mL in DMSO. However, I can see the formation of crystals which I can only assume are DMSO and that the drug is precipitating out of...

14 February 2021 5,633 1 View

Why am I getting inconsistent results from my ChIP PCR?

One of the times I performed this ChIP I got strong binding at the locus I amplified, but I have not been able to reproduce this result after many many tries. However, I did get the protein I am...

10 December 2020 7,279 1 View

For gene expression, is it better to average all the exons or is it better just to take the expression of a more stable region like the 3'UTR?

Some techniques involve averaging exon expression levels, but this can be a problem for genes that exhibit alternative splicing, so I'm wondering if there's a more stable region to take to get a...

22 June 2017 6,050 3 View

How does one test blood for anti-serotonin antibodies?

I have done research, but I am making a lot of inferences and I want to be sure I am correct. 1) Does one use ELISA? 2) Does one use serotonin, the patient's blood, and a biomarker to show if /...

17 February 2015 2,162 2 View

Are you aware of any studies that address validity of self-report attachment instruments?

I am a fan of the AAI and the developers on the CAI, so I need to counterbalance my positive bias with some critical questions about validity.

16 November 2013 5,733 3 View

Can the coefficient of variation (CV) of ANPP exceed 1 (i.e. 100%)?

I'm running a simulation on effects of changing mean and SD of rainfall on mean and CV of ANPP. At the most arid site I'm getting values of >100% CV for ANPP, when simulated mean rainfall is...

28 July 2013 814 8 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

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01 March 2021 3,905 5 View

Which group formation theory do you recommend for projects that aim to bring together entrepreneurs for training for regional development?

I would like to have ideas about which theories of group formation are suitable for entrepreneurial training projects, with the aim of creating bonds, building trust, and collaboration for...

28 February 2021 4,223 2 View

What is the most common number of epitopes in an multi-epitope Vaccine?

hi everyone, I'm interested in the field of vaccine design specifically multi-epitope cancer vaccines , I want to know if there is a minimum and maximum number of epitopes we could use in a...

27 February 2021 3,882 3 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

What is the optimum time and temperature of blanching and pre-frying potato for frozen fries?

I am helping a local home industry for developing frozen fries from their local farm. I want to know what is the optimum blanching and pre-frying temperature and time for processing. Please also...

23 February 2021 1,616 5 View

What to do with absence of fluorescence label in Fucci2a mouse-derived fibroblast culture?

Dear all, I have harvested fibroblast cells from R26p-Fucci2 mouse embryos (mouse line as described in this paper: https://dev.biologists.org/content/140/1/237) and cultured them on plastic,...

17 February 2021 3,368 2 View

How to disaggregate country data into city ones?

I'm using a number of indicators in my research which are not available at a sub-national level. Is there a reliable method for disaggregating the country data into city ones? (preferably with a...

16 February 2021 6,861 3 View

Which and How to use teaching strategies and procedures to effectively integrate the four skills while teaching English Language Online?

As an inexperienced teacher in the field of online teaching, I have the fear of unconsciously ignoring one of the skills while focusing on not ignoring the other! Therefore, I wish to make use of...

09 February 2021 5,101 4 View

Master's thesis ideas related to City Marketing and City Branding?

I have started collaborating with a small city that is planning to attract international talents from the capital city. The city municipality still doesn't know what audiences can fit better with...

09 February 2021 2,081 3 View