I want to sterilize my banana shoot tips with NaOCl in the initial stage of plant tissue culture in largescale. Plant culture tested (PCT) NaOCl (4%) in Hi-media is too costly (500 ml costs Rs. 1000/-), whereas Hi-AR grade 4% Sodium hypo-chloride costs only 1400/- for 5 lit. Can anybody advise me on the usage of AR grade chemical instead of PCT. Please share your experiences with respect to cost reduction in commercial banana tissue culture especially chemicals used in sterilization.
We use commercial bleach (Clorox brand). Depending on type of sterilization, we dilute it to 10 - 20 % v/v with D. H2O, and it is fairly effective.
Commercial bleach (contains about 6% sodium hypochlorite) commonly used disinfectants for plant tissue culture. It is prefer using 70% ethyl alcohol solution prior to treatment with bleach
surface sterilized with 70% ethanol for 30 seconds, submerged in sodium hydrochloride (Clorox) based on the result of sterilization experiment that must be perform using several concentrations and time periods. It is prefer using drops from tween 20 prior to treatment with sodium hydrochloride.
- Badges
- Science method