Ravindra Kumar Kurimella

8 Questions 35 Answers 0 Followers

Questions related from Ravindra Kumar Kurimella

In tissue culture, phenol exudation is high from immature tissues/explants or matured explants? Reasons please?

I am trying to standardize commercial tissue culture protocols for different conventional genotypes of India. In the process of experimentation, recorded very high phenol exudation from immature...

21 August 2023 3,669 1 View

Can I use Hi-AR grade Sodium hypo-chloride instead of Plant culture tested (PCT) NaOCl?

I want to sterilize my banana shoot tips with NaOCl in the initial stage of plant tissue culture in largescale. Plant culture tested (PCT) NaOCl (4%) in Hi-media is too costly (500 ml costs Rs....

01 January 2020 8,931 5 View

Can anybody suggest the in vitro media supplement to strengthen the banana shoots?

Hi everyone we are in the process of standardizing the micro-propagation protocol of culinary banana (ABB) for commercial scale production. But, unlike Grand Naine and other table varieties of...

27 June 2019 3,048 2 View

Can any one suggest best chemical/treatment to control snails in betelvine?

Betel vine plantations are highly infested with snails. They are eating both betel leaf and sesbania (Stake plant) leaves causing great loss to the farmers. Salt can use to control these types of...

20 October 2017 7,162 3 View

Can any body explain the reason behind the decrease in shoot length in gibberellin supplemented media (in vitro) as compared to control

After shoot proliferation stage,transferred my tagetes (marigold) micro-shoots to different GA (0.5, 1.0 and 2.0 mg/l) supplemented mediums. For other crops, it is a regular practice in our lab so...

01 July 2017 7,887 7 View

Can anybody give the reason for delayed rooting in liquid culture compared to solid or semi-solid media?

I am working on Tagetes spp(Marigold) tissue culture. Delayed rooting observed in liquid media containing 0.5 mg/l IBA with half MS compared to solid media having same composition. Although number...

10 October 2016 4,485 3 View

Which method is useful in observing nucleus (Vegetative & sperm) maturation in Tagetes (Marigold) pollen grain?

Hi, i want to correlate microspore development stage with flower bud size in Tagetes spp. (Marigold). I am currently using aceto-carmine stain for pollen staining, but i can find up to tetrad...

26 September 2015 9,074 4 View

Does anyone have an idea on double haploid production in Marigold/Chrysanthemum?

I want to develop DH in Marigold through another culture. Can anybody suggest the protocols available on Genotype selection, Media composition, Hormonal concentrations, stage of flower bud...

24 October 2014 9,327 2 View