What the error mean square (in a split plot design) is always higher for the main plot error as compared to the sub plot error ?

I know that in split plot design the main plot error is larger than the sub plot error. There is more error degree of freedom for sub plot as compared to main plot. But, in statistical analysis,...

14 December 2020 6,669 4 View

Which are the best methods used for isolation and purification of plant pathogenic fungi and bacteria ?

Best methods suitable for isolation and purification of plant pathogenic fungi and bacteria from plant and soil samples....Latest methods....Any special advantages....Procedure to be followed...etc.

28 November 2020 3,365 3 View

How health psychology is helpful to deal the daily life issues?

Psychological Impact of Health Disease on Human Behaviour. If anybody is interested they can join us.

10 August 2020 8,007 13 View

Does plasma therapy works for treatment of covid-19?

04 August 2020 8,186 11 View

In ansys CFD, simulation, Can we initialize two fluids kept in two adjacent compartment with two different temperatures?

Compartment wall temperature can easily initialzed with boundary conditions but if fluid inside it are at different temperatures?

03 February 2020 4,495 4 View

In Ansys CFD can we assign cell zone temperature conditions along with existing fluid/solid material conditions?

Cell zone conditions are about assigning a particular fluid or solid inside it, but it does not allow hot or cold conditions of fluid inside it. can we assign them in Ansys CFD simulation?

03 February 2020 5,199 4 View

How do we determine the position and orientation of a moving robot ??

How do we determine the position and orientation of a moving robot using IMU sensors such as MPU6050 or MPU9250 (i.e. accelerometer , gyroscope and magnetometer sensor data)??? kindly advise...

22 November 2019 7,825 5 View

Why overaging treament is required in continuous annealing line for production of dual phase steel?

Dual phase steel can be produced by directly quenching to room temperature from inter-critical temperature range. Then why overaging treatment is given in continious annealing line in steel...

09 September 2019 6,322 4 View

How to separate Adenosine and Inosine?

Hi everyone I have been trying to resolve adenosine and Inosine on HPLC column. The details are as follows: 1) Column dimension: 150mmX4.6mm, with 5 micron bead size. 2) Mobile phase: 20mM...

25 August 2019 3,420 3 View

Is Algae-based Bio-Fuel Practical for the Future?

Around the globe there is discussion going on that the algae are the feasible option for biofuel. I agree with them partially but, I think there is still long way. What is your opinion?

17 November 2018 9,520 16 View

Phenol-pH graphic?

We want to use phenol pH graphic to measure the pH of mediums before and after inhibitor application to cells. We have phenol absorbence values at 460, 560 and 720 nm wavelength. When we measure...

20 February 2021 7,700 3 View

Has anyone encountered any problems with abm good microRNA primers for quantification of mature miRNAs?

I've been trying to quantitate mature microRNAs in 293T cells with and without 'X'. 2ug of total RNA (isolated by Trizol method) is converted to cDNA using All-in one microRNA cDNA synthesis kit...

17 February 2021 8,986 2 View

Does anybody know the ways of homogeneous ripening of Mung bean crop?

Some crops (Mung bean) are matured at different times i.e., harvested at several times that's why the labour cost of the farmer is high. So, could you please, is there any ways or chemicals are...

12 February 2021 294 4 View

What is suitable temperature at reaction mixture of lipase activity assay?

I use para nitrophenyl palmitate substrate with tris buffer ph 8... i read many temperature for Yellow color of para nitro phenol production.. 37 and 50 and 40 at water bath ... I confused as i...

04 February 2021 9,100 5 View

How much could knowing the maturity level of an organization contribute to a given process?

The doubt is raised because there are several methodologies to estimate the degree of maturity of certain processes, such as computerization, communication, human resources, among others, and...

03 February 2021 4,753 7 View

Can you use MTS assay with cells cultured in media containing phenol red?

I am trying to run a cell viability assay with LN229, but these cells are cultured in DMEM which contain phenol red. Is the MTS assay sensitive to the additional of phenol red in the media? If so,...

03 February 2021 9,478 2 View

How to use plant root tissue for experimental analysis?

Hello everyone, I am working basically on plants (hydroponic culture). I want to estimate biochemicals and gene expression pattern of root tissues. My question is that how to harvest the tissues...

20 January 2021 4,443 3 View

Can anyone walk me through IHC/IF Staining of Cryosectioned Brain Tissues?

I'm looking to do some IHC/IF staining of X. laevis brains that have been frozen in OCT media for cryosectioning. I understand how to do the cryosectioning, but I am hoping for help/protocols that...

14 January 2021 9,752 2 View

How to Extract DNA from dried rhizomes, wood, galls etc. stored for a long time?

Dear Respectable Scientists, I have been trying to extract DNA from above mentioned sources using different CTAB/SDS Buffer Compositions and purifying agents but I am not able to extract DNA from...

12 January 2021 5,899 8 View

How to get phenol(BHT) back to organic phase from acidic aqueous layer?

07 January 2021 6,527 3 View