I would reproduce in my lab the buffer that facilitates binding of magnetic beads with nucleic acid. In according to DNA or RNA extraction using magnetic beads technology.
Dear Vincenzo,
Dynabeads M-280 or M-270 (invitrogen, Dynal) suggest the following binding and wash buffer (B&W):
2x concentrated:
10 mM Tris-HCl (pH 7.5), 1 mM EDTA, 2M NaCl, (optional) 0.1%Tween 20.
Adding an equal volume of biotinylated DNA or RNA gives an 1x binding buffer with a concentration of 1 M NaCl.
Hoping this is a good suggestion,
Maria
Many thanks Maria!
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