Can anyone tell me how a binding buffer for magnetic beads is made?

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Maria Sotirios Kaponi

Dear Vincenzo,

Dynabeads M-280 or M-270 (invitrogen, Dynal) suggest the following binding and wash buffer (B&W):

2x concentrated:

10 mM Tris-HCl (pH 7.5), 1 mM EDTA, 2M NaCl, (optional) 0.1%Tween 20.

Adding an equal volume of biotinylated DNA or RNA gives an 1x binding buffer with a concentration of 1 M NaCl.

Hoping this is a good suggestion,

Maria

Vincenzo Costantino

Many thanks Maria!