Do you know a protocol to extract DNA from Gram + bacteria in human feces with magnetic beads technology?

More Vincenzo Costantino's questions See All

Can anyone tell me how a binding buffer for magnetic beads is made?

I would reproduce in my lab the buffer that facilitates binding of magnetic beads with nucleic acid. In according to DNA or RNA extraction using magnetic beads technology.

03 April 2014 1,336 2 View

Can anyone help me with miRNA extraction with magnetic beads?

I am looking for a commercial kit that uses magnetic beads technology for miRNA extraction starting from whole blood, serum or tissue samples. Anybody could help me? Many thanks

02 March 2014 5,221 0 View

What is the best method to remove RNAlater from frozen tissue at -20?

It is sufficient to remove the tissue from RNAlater and put it in lysis solution?

02 March 2014 5,990 2 View

Can a pair of primer designed for a specific cDNA amplify also a genomic region?

In my lab there are primers for specific cDNA and I would like to know if they are useful to amplify a genomic region

01 February 2014 524 19 View

Are RNA viroids from plants studied like other viruses or do they need other strategies?

RNA viroids studies.

01 February 2014 6,005 2 View

What are the methods for evaluating methylation conversion?

Is a simple PCR sufficient?

31 December 2013 4,397 2 View

How is lysis buffer for DNA extraction made?

I would like to make my own lysis buffer for DNA extraction from blood and tissues

10 November 2013 7,569 3 View

What is the average yield (ng/ul) after a DNA extraction using magnetic beads starting from plant samples (example, 10 mg of leaf tissue)?

I have obtained a yield of 20-30 ng/ul eluiting with 100 ul of distilled water.

10 November 2013 4,871 2 View

Have you ever tried the TATAA Universal DNA spike kit to detect any contaminants in your genomic DNA sample?

The kit is based on a Real Time PCR reaction and differences in Delta Cq. Could you give me any feedback?

09 October 2013 8,348 0 View

I would like to know if 1,2% gel is good for running the genomic DNA. Can you help me?

In past I have seen that gDNA runs well on 0,8 gel (home made gel agarose) but with precast gel I haven't had the possibility of choosing the right percent of agarose.

09 October 2013 9,395 8 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How can I inoculate a lyophilized anaerobic bacteria into the culture medium without an anaerobic chamber?

There are anaerobic gas pak jars and environment providers in the laboratory that provide anaerobic environmental conditions for the growth of anaerobic bacteria. However, there is no anaerobic...

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Why do paired electrons atoms produce no magnetic field lines?

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03 August 2024 3,927 2 View

How to correctly use the UTE and ZTE pulse sequences in Bruker's ParaVision software?

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Mossbauer spectroscopy Co0.6Mn0.4Fe2O4 MNPs?

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How does the Retention time effect the Biogas Production?

List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?

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Question about MHB (Mycorrhiza Helper Bacteria) isolation technique??

What are the most effective isolation techniques for obtaining MHB?? Is there are best approach techniques? I keep struggling in obtaining it.

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How to extract RNA from neurons?

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Pink bacterial colonies?

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What does negative readings in spectrophotometer denote. Was testing TSS content of bacterial cultures?

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Ewa Stepien

Hi Vincenzo,

BioMerieux system (semi-automated) based on Boom's technology is dedicated to feces. Some people use it in clinical labs.

Vincenzo Costantino

Hi Ewa,

many thanks for your answer. In Masmec Biomed, we just produce automated workstations for DNA, RNA and miRNA extraction. I am looking only for a kit to use for our workstation.

Jay Siddharth

http://www.mobio.com/soil-dna-isolation/powersoil-htp-96-well-soil-dna-isolation-kit.html

optimised for Kingfisher and epimotion

Alexander Sasha Vlassov

am1840 MagMax total nucleic acid isolation kit

Falak Azzam

AM1840 MagMax Total nucleic acid isolation kit from Ambion (ThermoFisher Sientific) gives a spectacular results.