Do you know a protocol to extract DNA from Gram + bacteria in human feces with magnetic beads technology?

More Vincenzo Costantino's questions See All

Can anyone tell me how a binding buffer for magnetic beads is made?

I would reproduce in my lab the buffer that facilitates binding of magnetic beads with nucleic acid. In according to DNA or RNA extraction using magnetic beads technology.

03 April 2014 1,169 2 View

Can anyone help me with miRNA extraction with magnetic beads?

I am looking for a commercial kit that uses magnetic beads technology for miRNA extraction starting from whole blood, serum or tissue samples. Anybody could help me? Many thanks

02 March 2014 5,143 0 View

What is the best method to remove RNAlater from frozen tissue at -20?

It is sufficient to remove the tissue from RNAlater and put it in lysis solution?

02 March 2014 5,905 2 View

Can a pair of primer designed for a specific cDNA amplify also a genomic region?

In my lab there are primers for specific cDNA and I would like to know if they are useful to amplify a genomic region

01 February 2014 430 19 View

Are RNA viroids from plants studied like other viruses or do they need other strategies?

RNA viroids studies.

01 February 2014 5,915 2 View

What are the methods for evaluating methylation conversion?

Is a simple PCR sufficient?

31 December 2013 4,305 2 View

How is lysis buffer for DNA extraction made?

I would like to make my own lysis buffer for DNA extraction from blood and tissues

10 November 2013 7,472 3 View

What is the average yield (ng/ul) after a DNA extraction using magnetic beads starting from plant samples (example, 10 mg of leaf tissue)?

I have obtained a yield of 20-30 ng/ul eluiting with 100 ul of distilled water.

10 November 2013 4,657 2 View

I would like to know if 1,2% gel is good for running the genomic DNA. Can you help me?

In past I have seen that gDNA runs well on 0,8 gel (home made gel agarose) but with precast gel I haven't had the possibility of choosing the right percent of agarose.

09 October 2013 9,292 8 View

Have you ever tried the TATAA Universal DNA spike kit to detect any contaminants in your genomic DNA sample?

The kit is based on a Real Time PCR reaction and differences in Delta Cq. Could you give me any feedback?

09 October 2013 8,269 0 View

How do I determine the degree of polymerization of PEG 400 based on NMR integration?

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Do you know any MR image dataset?

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What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

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How to include original NMR data in a manuscript?

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What is the exact mechanism of the antimicrobial effect of local anesthetics?

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Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

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Advice on growing lactobacillus species in the lab?

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How to improve the buffer capacity of sea water?

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What are the best cations for preparing competent cells, Ca2+ or Mg2+?

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Would using an asRNA for repressing transcription of a high expression Listeria monocytogenes non-coding RNA harbored in a plasmid be a viable option?

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Ewa Stepien

Hi Vincenzo,

BioMerieux system (semi-automated) based on Boom's technology is dedicated to feces. Some people use it in clinical labs.

Vincenzo Costantino

Hi Ewa,

many thanks for your answer. In Masmec Biomed, we just produce automated workstations for DNA, RNA and miRNA extraction. I am looking only for a kit to use for our workstation.

Jay Siddharth

http://www.mobio.com/soil-dna-isolation/powersoil-htp-96-well-soil-dna-isolation-kit.html

optimised for Kingfisher and epimotion

Alexander Sasha Vlassov

am1840 MagMax total nucleic acid isolation kit

Falak Azzam

AM1840 MagMax Total nucleic acid isolation kit from Ambion (ThermoFisher Sientific) gives a spectacular results.