I am looking for a commercial kit that uses magnetic beads technology for miRNA extraction starting from whole blood, serum or tissue samples. Anybody could help me? Many thanks
I am looking for a commercial kit with magnetic beads to extract DNA from GRAM+ in human feces.
08 September 2014 5,964 5 View
I would reproduce in my lab the buffer that facilitates binding of magnetic beads with nucleic acid. In according to DNA or RNA extraction using magnetic beads technology.
03 April 2014 1,336 2 View
It is sufficient to remove the tissue from RNAlater and put it in lysis solution?
02 March 2014 5,990 2 View
In my lab there are primers for specific cDNA and I would like to know if they are useful to amplify a genomic region
01 February 2014 524 19 View
RNA viroids studies.
01 February 2014 6,005 2 View
Is a simple PCR sufficient?
31 December 2013 4,397 2 View
I would like to make my own lysis buffer for DNA extraction from blood and tissues
10 November 2013 7,569 3 View
I have obtained a yield of 20-30 ng/ul eluiting with 100 ul of distilled water.
10 November 2013 4,871 2 View
The kit is based on a Real Time PCR reaction and differences in Delta Cq. Could you give me any feedback?
09 October 2013 8,348 0 View
In past I have seen that gDNA runs well on 0,8 gel (home made gel agarose) but with precast gel I haven't had the possibility of choosing the right percent of agarose.
09 October 2013 9,395 8 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
How to design VN primer to attach with universal reverse primer
05 August 2024 2,116 3 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?
03 August 2024 7,829 0 View