Vincenzo Costantino

14 Questions 24 Answers 0 Followers

Questions related from Vincenzo Costantino

Do you know a protocol to extract DNA from Gram + bacteria in human feces with magnetic beads technology?

I am looking for a commercial kit with magnetic beads to extract DNA from GRAM+ in human feces.

09 September 2014 5,922 5 View

Can anyone tell me how a binding buffer for magnetic beads is made?

I would reproduce in my lab the buffer that facilitates binding of magnetic beads with nucleic acid. In according to DNA or RNA extraction using magnetic beads technology.

04 April 2014 1,247 2 View

What is the best method to remove RNAlater from frozen tissue at -20?

It is sufficient to remove the tissue from RNAlater and put it in lysis solution?

03 March 2014 5,961 2 View

Can anyone help me with miRNA extraction with magnetic beads?

I am looking for a commercial kit that uses magnetic beads technology for miRNA extraction starting from whole blood, serum or tissue samples. Anybody could help me? Many thanks

03 March 2014 5,193 0 View

Can a pair of primer designed for a specific cDNA amplify also a genomic region?

In my lab there are primers for specific cDNA and I would like to know if they are useful to amplify a genomic region

02 February 2014 482 19 View

Are RNA viroids from plants studied like other viruses or do they need other strategies?

RNA viroids studies.

02 February 2014 5,978 2 View

What are the methods for evaluating methylation conversion?

Is a simple PCR sufficient?

01 January 2014 4,359 2 View

What is the average yield (ng/ul) after a DNA extraction using magnetic beads starting from plant samples (example, 10 mg of leaf tissue)?

I have obtained a yield of 20-30 ng/ul eluiting with 100 ul of distilled water.

11 November 2013 4,737 2 View

How is lysis buffer for DNA extraction made?

I would like to make my own lysis buffer for DNA extraction from blood and tissues

11 November 2013 7,526 3 View

Does anyone know how a microplate reader (for ELISA experiment) is made?

I would like to know all the components: filters, mirror, etc.

10 October 2013 8,499 1 View

Have you ever tried the TATAA Universal DNA spike kit to detect any contaminants in your genomic DNA sample?

The kit is based on a Real Time PCR reaction and differences in Delta Cq. Could you give me any feedback?

10 October 2013 8,318 0 View

Different lysis time according to different tissues?

I have to lyse different tissues (brain, liver, spleen and kidney) with enzymatic and mechanical system to perform DNA extraction. Could you tell me if are there different lysis time according...

10 October 2013 9,285 1 View

I would like to know if 1,2% gel is good for running the genomic DNA. Can you help me?

In past I have seen that gDNA runs well on 0,8 gel (home made gel agarose) but with precast gel I haven't had the possibility of choosing the right percent of agarose.

10 October 2013 9,363 8 View

Who knows the right composition both of the lysis buffer and the magnetic beads beads buffer for DNA extraction?

Magnetic beads for DNA extraction

07 July 2013 5,651 0 View