14 Questions 24 Answers 0 Followers
Questions related from Vincenzo Costantino
I am looking for a commercial kit with magnetic beads to extract DNA from GRAM+ in human feces.
09 September 2014 5,930 5 View
I would reproduce in my lab the buffer that facilitates binding of magnetic beads with nucleic acid. In according to DNA or RNA extraction using magnetic beads technology.
04 April 2014 1,259 2 View
It is sufficient to remove the tissue from RNAlater and put it in lysis solution?
03 March 2014 5,966 2 View
I am looking for a commercial kit that uses magnetic beads technology for miRNA extraction starting from whole blood, serum or tissue samples. Anybody could help me? Many thanks
03 March 2014 5,200 0 View
In my lab there are primers for specific cDNA and I would like to know if they are useful to amplify a genomic region
02 February 2014 491 19 View
RNA viroids studies.
02 February 2014 5,985 2 View
Is a simple PCR sufficient?
01 January 2014 4,366 2 View
I have obtained a yield of 20-30 ng/ul eluiting with 100 ul of distilled water.
11 November 2013 4,742 2 View
I would like to make my own lysis buffer for DNA extraction from blood and tissues
11 November 2013 7,532 3 View
I would like to know all the components: filters, mirror, etc.
10 October 2013 8,505 1 View
The kit is based on a Real Time PCR reaction and differences in Delta Cq. Could you give me any feedback?
10 October 2013 8,326 0 View
I have to lyse different tissues (brain, liver, spleen and kidney) with enzymatic and mechanical system to perform DNA extraction. Could you tell me if are there different lysis time according...
10 October 2013 9,294 1 View
In past I have seen that gDNA runs well on 0,8 gel (home made gel agarose) but with precast gel I haven't had the possibility of choosing the right percent of agarose.
10 October 2013 9,371 8 View
Magnetic beads for DNA extraction
07 July 2013 5,657 0 View