What are the methods for evaluating methylation conversion?

More Vincenzo Costantino's questions See All

Do you know a protocol to extract DNA from Gram + bacteria in human feces with magnetic beads technology?

I am looking for a commercial kit with magnetic beads to extract DNA from GRAM+ in human feces.

08 September 2014 5,860 5 View

Can anyone tell me how a binding buffer for magnetic beads is made?

I would reproduce in my lab the buffer that facilitates binding of magnetic beads with nucleic acid. In according to DNA or RNA extraction using magnetic beads technology.

03 April 2014 1,169 2 View

Can anyone help me with miRNA extraction with magnetic beads?

I am looking for a commercial kit that uses magnetic beads technology for miRNA extraction starting from whole blood, serum or tissue samples. Anybody could help me? Many thanks

02 March 2014 5,143 0 View

What is the best method to remove RNAlater from frozen tissue at -20?

It is sufficient to remove the tissue from RNAlater and put it in lysis solution?

02 March 2014 5,905 2 View

Can a pair of primer designed for a specific cDNA amplify also a genomic region?

In my lab there are primers for specific cDNA and I would like to know if they are useful to amplify a genomic region

01 February 2014 430 19 View

Are RNA viroids from plants studied like other viruses or do they need other strategies?

RNA viroids studies.

01 February 2014 5,915 2 View

How is lysis buffer for DNA extraction made?

I would like to make my own lysis buffer for DNA extraction from blood and tissues

10 November 2013 7,472 3 View

What is the average yield (ng/ul) after a DNA extraction using magnetic beads starting from plant samples (example, 10 mg of leaf tissue)?

I have obtained a yield of 20-30 ng/ul eluiting with 100 ul of distilled water.

10 November 2013 4,657 2 View

I would like to know if 1,2% gel is good for running the genomic DNA. Can you help me?

In past I have seen that gDNA runs well on 0,8 gel (home made gel agarose) but with precast gel I haven't had the possibility of choosing the right percent of agarose.

09 October 2013 9,292 8 View

Have you ever tried the TATAA Universal DNA spike kit to detect any contaminants in your genomic DNA sample?

The kit is based on a Real Time PCR reaction and differences in Delta Cq. Could you give me any feedback?

09 October 2013 8,269 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Stéphane Roche

If you speak about the conversion of 5methylcytosine by bisulfite conversion, you might performed methyl specific PCR or sequencing. Both works well but both need some experiments to work out. For methyl specific PCR, you need to perform one PCR for methyled DNA and one PCR for unmethyled DNA.

Vincenzo Costantino

Thanks a lot Stephane! Your suggestions will be precious!